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Karpuk I.Y.
In vivо diagnosing of allergy in patients with intolerance to chrome

The majority of panels for the detection of allergy to metals include solutions of their salts in concentrations about one g/100 ml or mol/l. However, they can differ in the solvents used in their production (water, alcohol, oils, vaseline), anions (chlorides, sulfates, nitrates, etc.). It causes a certain variability of the received results. But as panels include mainly the substances possessing the highest allergenic potential, in principle the obtained results have a certain overlapping that allows the use of these panels for clinical purposes, although the reliability of diagnosing results on these or those concentrations of metal salts remains unclear.
Thus, there is a necessity for the determination of non-toxic concentrations of metal salts for skin tests.
The objective of this research was to establish optimum concentration of CrCl3 salt as an allergen by means of application skin tests with solutions of CrCl3 salt of different concentration for identification of sensitization to it.
The method of patch testing with a solution of CrCl3 salt for revealing sensitization of an organism to chrome has been developed and clinically tested.
The following groups of patients have been surveyed: with intolerance to dental prosthetic materials, having fixed orthopedic designs of artificial prostheses (n=43); the control group consisted of almost healthy 40 persons.
It has been established that the optimal concentration of CrCl3 salt solution for patch tests is three-percent. Concentrations of 0,5 and 1% of CrCl3 salt solution are insufficient for more reliable revealing of an allergy to chrome in patients with intolerance to tooth artificial prostheses. On 5% concentration of CrCl3 salt solution there is a considerable quantity of positive reactions in the control group that allows to consider the given concentration as toxic.
The suggested approach to the diagnosing of allergy to metals helps to avoid false-positive reactions which have resulted from the toxicity of salt, used as an allergen.
Key words: allergy, concentration of chrome salt, skin tests, diagnosing.


1.    Brasch J, Geier J. Patch test results in schoolchildren. Results from the Information Network of Departments of Dermatology (IVDK) and the German Contact Dermatitis Research Group (DKG). Contact Dermatitis. 1997 Dec;37(6):286-93.
2.    Schnuch A, Uter W, Geier J, Gefeller O. Epidemiology of contact allergy: an estimation of morbidity employing the clinical epidemiology and drug-utilization research (CE-DUR) approach. Contact Dermatitis. 2002 Jul;47(1):32-9.
3.    Moiseichik PN, Skep'ian NA. Formirovanie allergicheskikh reaktsii v zavisimosti ot sochetannogo vozdeistviia gaptenov [Formation of allergic reactions depending on the combined effects of haptens]. V: Sostoianie stomatologicheskoi pomoshchi naseleniiu i puti ee usovershenstvovaniia v usloviiakh perekhodnoi ekonomiki: materialy 3 s"ezda stomatologov Belarusi. Minsk, RB; 1997. р. 213-214.
4.    Moiseichik PN, sost, Skep'ian NA, sost, Velichko LS, sost, Naumovich SA, sost. Prognozirovanie, diagnostika i profilaktika allergicheskikh reaktsii v ortopedicheskoi stomatologii [Forecasting, diagnostics and prophylaxis of allergic reactions in an orthopedic odontology]: metod. rek. Minsk, RB; 1999. 23 р.
5.    Titov PL. Otsenka sensibilizatsii organizma k ionam metallov in vivo u lits s predpolagaemym neblagopriiatnym lokal'nym vozdeistviem dental'nykh splavov [Assessment of a sensibilization of an organism to ions of in vivo metals at persons with alleged adverse local effect the dentalnykh of alloys]. Belorusskii meditsinskii zhurnal. 2004;(4):89-92.
6.    Fedorovich SV, Moiseichik PN, Skep'ian NA, Sokolov SM. Allergiia v stomatologicheskoi praktike [Allergy in stomatologic practice]. Baranovichi, RB; 2001. 182 р.
7.    Lebedev KA, Mitronin AV, Poniakina ID. Neperenosimost' zuboproteznykh materialov [Intolerance of dentoprosthetic materials]. Moscow, RF: Librokom; 2010. 208 р.
8.    Gozhaia LD. Allergicheskie i toksiko-khimicheskie stomatity, obuslovlennye materialami zubnykh protezov [The allergic and toksiko-chemical stomatites caused by materials of dentures]: metod. posobie dlia vrachei-stomatologov. Moscow, RF; 2000. 31 р.
9.    Poluev VI, Leont'ev VK, Shestakov VT. Professional'nye riski i professional'naia otvetstvennost' rabotnikov stomatologicheskogo profilia [Professional risks and professional responsibility of workers of a stomatologic profile]. Stomatologiia dlia vsekh. 2001;(2):40-3.
10.    Mallo Pérez L, Díaz Donado C. Intraoral contact allergy to materials used in dental practice. A critical review. Med Oral. 2003 Nov-Dec;8(5):334-47.
11.    Novikov DK, Sergeev YuV, Novikov PD. Lekarstvennaia allergiia [Medicinal allergy]. Moscow, RF: Nats. akad. mikologii; 2001. 313 р.
12.    Brasch J, Geier J, Henseler T. Evaluation of patch test results by use of the reaction index. An analysis of data recorded by the Information Network of Departments of Dermatology (IVDK). Contact Dermatitis. 1995 Dec;33(6):375-80.
13.    Brasch J, Henseler T. The reaction index: a parameter to assess the quality of patch test preparations. Contact Dermatitis. 1992 Sep;27(3):203-4.

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