Sergeyeva S.P., Breslavich I.D, Erofeyeva L.M., Gultyayev M.M.
Dynamics and prognostic value of CD3CD95 lymphocytes, soluble Fas receptor and Fas ligand levels in peripheric blood after ischemic insult
Abstract.
Objectives. To investigate the dynamics CD3CD95 lymphocytes, soluble Fas receptor (sFas) and soluble Fas ligand (sFasL) levels in peripheric blood of patients after ischemic insult, the correlation between them, as well as the relationship between these levels and the neurologic manifestations severity.
Material and methods. In all 60 patients were studied, 25 of them were healthy controls. By means of ELISA sFas and sFasL plasma levels were detected. Fas protein (CD95) expression on CD3 lymphocytes surfaces was detected using flow cytometry. To objectivize the estimation of the patients’ severity condition, focal neurologic deficiency expressiveness the point scale of stroke assessment of National Institute of Health (NIH) was used.
Results. The level of sFasL and sFasL/sFas ratio correlated with the severity of patients’ condition after ischemic insult and neurologic disorders (p<0,05).
Conclusions. The changes of plasma level of sFasL and sFasL/sFas ratio are prognostic markers and may be used in clinical practice for determining the management of patients with ischemic insult. Fas induced apoptosis mechanisms seem to be a worthwhile target for the search of new ways in diagnosing, assessment of prognosis and treatment efficacy of patients suffering from ischemic insult.
Key words: apoptosis, ischemic insult, lymphocytes.
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