Ivanova S.V.
Proteolysis system parameters of synovial fluid as diagnostic markers of some forms of arthrites
Education Establishment "Vitebsk State Order of Peoples’ Friendship Medical University", Republic of Belarus
Abstract.
In this article the analysis of certain parameters of proteolysis system of synovial fluid in patients with rheumatoid, reactive and psoriatic arthrites and their diagnostic value has been made. It has been experimentally determined that in synovial fluid the lowest level of 1-antiproteinase inhibitor content was observed in patients with rheumatoid arthritis. It has also been established, that the level of I1-antiproteinase inhibitor content in synovial fluid less than 0,41 g/l can be used as a diagnostic sign of rheumatoid arthritis. The lowest value of proteolysis index in synovial fluid was observed in the group of patients with psoriatic arthritis. The determination of proteolysis index in synovial fluid proved to be effective for the diagnosis of psoriatic arthritis: values of the given parameter less than 1,80 were a diagnostic marker of psoriatic arthritis. The scheme of using blood serum fluorescence and proteolysis activity parameters of synovial fluid for the differential diagnosis of rheumatoid, reactive and psoriatic arthrites in combination with traditional laboratory methods has been suggested.
Key words: proteolysis, synovial fluid, diagnostic value.
References
1. Nikandrov VN, Pyzhova NS. Proteoliz kak universal'nyi me-khanizm reguliatsii biokhimicheskikh i biologicheskikh protsessov. Diskus-sionnye aspekty [Proteolysis as universal mechanism of a regulation of biochemical and biological processes. Debatable aspects]. Izvestiia NAN Belarusi. Ser. med. nauk. 2008;(1):4–22.
2. Turk B. Targeting proteases: successes, failures and future prospects. Nat Rev Drug Discov. 2006 Sep;5(9):785-99.
3. Hollenberg MD, Oikonomopoulou K, Hansen KK, Saifeddine M, Ramachandran R, Diamandis EP. Kallikreins and proteinase-mediated signaling: proteinase-activated receptors (PARs) and the pathophysiology of inflammatory diseases and cancer. Biol Chem. 2008 Jun;389(6):643-51.
4. Han N, Jin K, He K, Cao J, Teng L. Protease-activated receptors in cancer: a systematic review. Oncol Lett. 2011 Jul;2(4):599-608.
5. Chow AK, Cena J, Schulz R. Acute action and novel target of matrix metalloproteinases in the heart and vasculature. Br J Pharmacol. 2007 Sep;152(2):189-205.
6. Koriakina EV, Belova SV. Osobennosti patogeneticheskikh mekha-nizmov endogennoi intoksikatsii u bol'nykh revmatoidnym artritom [Features of pathogenetic mechanisms of endogenic intoxication at patients with a pseudorheumatism]. Nauchno-prakticheskaia revmatologiia. 2001;(1):21–9.
7. Murphy G, Knäuper V, Atkinson S, Butler G, English W, Hutton M, Stracke J, Clark I. Matrix metalloproteinases in arthritic disease. Arthritis Res. 2002;4 Suppl 3:39-49.
8. Martel-Pelletier J, Welsch DJ, Pelletier JP. Metalloproteases and inhibitors in arthritic diseases. Best Pract Res Clin Rheumatol. 2001 Dec;15(5):805-29.
9. Turna AA. Matriksnye metalloproteinazy v razvitii destruk-tivnykh protsessov pri revmatoidnom artrite [Matriksny metalproteinases in development of destructive processes at a pseudorheumatism]. Nauchno-prakticheskaia revmatologiia. 2010;(3):59–64.
10. Firestein GS. Etiology and pathogenesis of rheumatoid arthritis. Ruddy S, Harris ED, Sledge CB, Sergent JS, Budd RC. Kell’s Textbook of Rheumatology. Philadelphia: WB Sounders; 2001. Part 9. р. 1035-1086.
11. Firestein GS. Inhibiting inflammation in rheumatoid arthritis. N Engl J Med. 2006 Jan;354(1):80-2.
12. Erlanger BF, Kokowsky N, Cohen W. The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys. 1961 Nov;95:271-8.
13. Khvatov VB, Belova TA. Uskorennyi metod opredeleniia osnovnykh ingibitorov proteinaz v plazme krovi cheloveka [The accelerated method of definition of the main inhibitors of proteinases in a blood plasma of the person]: metod. rek. Moscow, RF; 1981. 16 р.
14. Rebrova OYu. Statisticheskii analiz meditsinskikh dannykh. Primenenie paketa prikladnykh programm Statistica [Statistical analysis of medical data. Application of a package of the applied Statistica programs]. Moscow, RF: MediaSfera; 2003. 312 р.
15. Menshikov VV. O klinicheskoi tsennosti laboratornykh issledovanii [About the clinical value of laboratory researches]. Klinicheskaia laboratornaia diagnostika. 1996;(5):4–12.
16. Ivanova SV, Kirpichenok LN. Diagnosticheskie i prognosticheskie vozmozhnosti opredeleniia osnovnykh pokazatelei sistemy proteoliza i intensivnosti fluorestsentsii v syvorotke krovi pri artritakh [Diagnostic and prognostic opportunities of definition of the main indicators of system of a proteolysis and intensity of fluorescence in blood serum at arthritises]. Laboratornaia diagnostika. Vostochnaia Evropa. 2012;(3):90–8.
