S.S. Osochuk, S.V. Buyanova, A.F. Martsinkevich
A method for estimating the probability of atorvastatin and its metabolically active derivatives association with low density and very low density lipoproteins
Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University», Vitebsk, Republic of Belarus
Abstract.
In this paper a method for estimating the probability of atorvastatin and its active derivatives association with blood lipoprotein complexes is proposed. The investigation included 16 healthy people and 29 patients with ischaemic heart disease (IHD). Blood was taken in 2 hours after the administration of atorvastatin in the dose of 80 mg. Blood lipoprotein complexes were isolated by ultracentrifugation. The levels of cholesterol, triacylglycerols, protein, atorvastatin and its metabolically active derivatives in native lipoprotein complexes were determined using photometric methods of analysis and high performance liquid chromatography. The amount of atorvastatin and its active derivatives associated with lipoprotein complexes in patients with IHD and healthy people were subjected to cluster analysis. The resulting two clusters were not significantly different in the quantity of atorvastatin and its derivatives in HDL, while one cluster, that included patients with IHD had an increased amount of atorvastatin and its derivatives in LDL and VLDL. To determine the predictors of atorvastatin and its active derivatives association with lipoprotein complexes the method of logistic regression was used. According to the obtained model, a statistically significant indicator reflecting the possibility of atorvastatin and its derivatives association with lipoprotein complexes is the amount of HDL cholesterol. When HDL cholesterol equals 1 mmol/l the probability of the increase of atorvastatin and its derivatives content in the composition of LDL/VLDL is 0,93%, when the amount of HDL cholesterol equals 0,5 mmol/l this probability increase reaches 18,83%, when the concentration of HDL cholesterol is 0,1 mmol/l, the probability will be 75,1%. On the basis of the analysis of HDL cholesterol indicators, the proposed method allows to determine the probability of the binding of atorvastatin and its active derivatives with LDL and VLDL with the accuracy of 84,8%, specificity of 87,5% and sensitivity of 77,7%.
Key words: atorvastatin, lipoprotein complexes, HDL cholesterol.
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