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Yarotskaya N.N.
Comparative morphometric evaluation of changes in the liver in experimental purulent peritonitis against the background of metabolic correction application
Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University», Vitebsk, Republic of Belarus

Vestnik VGMU. 2015;14(6):25-31.

Abstract.
Objectives. To study morphofunctional changes of the liver in experimental purulent peritonitis at the background of the application of the metabolic preparations «Citoflavin» and «Neoton».
Material and methods. The experiment was conducted on 55 rabbits. Purulent peritonitis was modelled by intraabdominal introduction of the aerobic and anaerobic mixture of E.coli and B.fragilis. In 6 hours after the initiation of peritonitis surgical treatment was carried out with the use during 5 days in the postoperative period of the metabolic preparations «Citoflavin», containing amber acid or «Neoton», containing creatine phosphate. Animals were brought out of the experiment in 6 hours after the initiation of peritonitis, and also on the 1st, 3rd, 5th days after the operation. Specimens of the liver served as the material for the morphological study. After fixing and standard histological processing and paraffin embedding we prepared serial slides stained with hematoxylin-eosin. Morphometric assessment of the slides was carried out with the use of Avtandilov`s morphometric grid.
Results. The obtained data testified to the degenerative changes in the liver tissue, the disturbance of an intraorganic blood flow, inflammatory polymorphocellular infiltration, tissue necrobiosis and necrosis development in experimental purulent peritonitis. The preparation containing amber acid in comparison with the preparation of creatine phosphate promoted faster regeneration processes with the restoration of the liver parenchyma structural and functional properties.
Conclusions. The initiation of experimental purulent peritonitis already in 6 hours leads to the morphofunctional changes development in the liver with dystrophic changes of hepatocytes, necrosis of hepatic cells, the disturbance of blood circulation, inflammatory infiltration of the parenchyma. The use of metabolic preparations in the postoperative period is followed by the decrease in expressiveness of destructive changes in the liver parenchyma with the increase of adaptation and regeneration processes.
Key words: experimental generalized purulent peritonitis, liver cytochrome c, citoflavin, neoton.

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