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DOI: https://doi.org/10.22263/2312-4156.2017.1.111

Pinkevych V.A., Kyslychenko A.A., Novosel E.N., Kyslychenko V.S.
The study of Parmelia thalluses polysaccharides
National University of Pharmacy, Kharkov, Ukraine

Vestnik VGMU. 2017;16(1):111-116.

Abstract.
Objectives. To study polysaccharides of  Parmelia thalluses.
Material and methods. Crushed Parmelia thalluses were the object of the study. The extraction of the sum of water-soluble polysaccharides from the plant raw material was carried out by hot water. Precipitation reaction by 96% ethanol was used to detect polysaccharides. Quantitative determination of the sum of water-soluble polysaccharides in Parmelia thalli was made by the gravimetric method. Separate fractions of polysaccharides were isolated from the defatted extraction cake. The fraction of water-soluble polysaccharides (WSPS) was obtained by hot water extraction. Pectin was extracted using the mixture of 0,5% solutions of oxalic acid and ammonium oxalate (1:1), and hemicellulose A (HC A) and hemicellulose B (HC B) – by means of 7% solution of sodium hydroxide.
Results. On adding to the aqueous extract of Parmelia thalluses the threefold volume of 96% ethanol the brown amorphous precipitate formed, which confirmed the presence of polysaccharides in the studied extract. As a result of the quantitative determination of the sum of water-soluble polysaccharides it was found, that their content in Parmelia thalluses made up 10,95±0,35%. On fractionation the yield of different fractions of polysaccharides was the following: WSPS – 8,90±0,30%, pectin – 12,32±0,42%, HC A – 14,67±0,47%, HC B – 8,47±0,28%.
Conclusions. The content of the sum of water-soluble polysaccharides in Parmelia thalluses was determined as a result of the conducted studies. For the first time individual fractions of polysaccharides were isolated and their quantitative content was determined. The results obtained testify to the prospects of further study of polysaccharide complexes of Parmelia thalli in order to create new effective phytoremedies.
Key words: lichen, parmelia, polysaccharides, pectin, hemicellulose.

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Information about authors:
Pinkevych V.A. – the fourth - year pharmaceutical student, National University of Pharmacy;
Кyslychenko A.A. – Candidate of Pharmaceutical Sciences, associate professor of the Chair of Pharmacognosy, National University of Pharmacy;
Novosel E.N. – Candidate of Pharmaceutical Sciences, associate professor of the Chair of Chemistry of Natural Compounds, National University of Pharmacy;
Кyslychenko V.S. – Doctor of Pharmaceutical Sciences, professor, head of the Chair of Chemistry of Natural Compounds, National University of Pharmacy.

Correspondence address: Ukraine, 61168, Kharkov, 4 Valentinovskaya str., National University of Pharmacy, Chair of Chemistry of Natural Compounds. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Viktoriya S. Кyslychenko.
                                                     

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