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Pabiarzhyn V.V.
Changes in the expression of markers in the glioma tissue of rats with ascariasis
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus

Vestnik VGMU. 2019;18(4):74-80.

Objectives. To study the effect of Ascaris invasion in the dose of 40 Ascaris suum eggs per gram of animal body weight on the expression of glioma markers in the experiment, depending on the observation period.
Material and methods. The experiment was carried out on female Wistar rats and consisted of 2 experimental series. Experimental animals after modelling the tumor of rat C6 glioma in situ were divided into 8 groups of 10 animals each. The first series of the experiment included animals of groups 1-4 from which the material was taken on the 14th, 21st, 28th, 35th days of tumor development, respectively, to obtain the results in «clean» tumor of rat C6 glioma in situ. The second series included animals of groups 5-8, the study of their material was carried out to assess the impact of Ascaris invasion on the studied parameters depending on the time from the beginning of infection. The material of all groups of animals (tumor, the liver, the lungs, the brain) was used for macroscopic, histological, and immunohistochemical analyses with the purpose of determining glial fibrillar acid protein (GFAP), S 100, proliferative activity marker Ki-67.
Results. It has been revealed that A. suum invasion in the dose of 40 eggs per gram of animal body weight 2.16 times increases GFAP expression in the biopsy specimens of the tumor tissue of rat C6 glioma in situ by the 7th day of invasion development; the expression of S 100 by the 7th day of invasion development – 2.53 times, by the 14th day after infection – 2.05 times; the proliferative activity index Ki 67 by the 7th day of invasion development – 2.28 times, by the 14th day after infection – 2.22 times.
Conclusions. The obtained data show that Ascaris invasion in the dose of 40 eggs per 1 gram of animal body mass increases the expression of GFAP, S 100 and proliferative activity marker Ki-67 in the tissues of rat C6 glioma in situ during the period of high biological activity of the parasite.
Key words: rat, glioma, immunohistochemical markers, GFAP, S 100, Ki-67, ascariasis.


1. Dolbin DA, Lutfullin MKh. The prevalence of asacaridosis in humans, age and demographic dynamics. Uchen Zap Kazan Gos Akad Veterinar Meditsiny im NE Baumana. 2015;222(2):83-5. (In Russ.)
2. Toygombaeva VS. Intestinal parasitic diseases of the population of the Republic of Kyrgyzstan. Med Parazitologiia. 2009;(2):31-3. (In Russ.)
3. Malyutina TA. Relationships in the parasite-host system: biochemical and physiological aspects of adaptation (retrospective review). Ros Parazitol Zhurn. 2008;(1):24-40. (In Russ.)
4. Hopkin J. Immune and genetic aspects of asthma allergy and parasitic worm infections evolutionary links. Parasite Immunol. 2009 May;31(5):267-73. doi:
5. Smith PJ, Theis B, McCartney S, Brown M. Helminths: an unrecognised disease burden prevalent among migrants in the gastroenterology clinic. Frontline Gastroenterol. 2011 Apr; 2(2):124-9. doi:
6. Ershova IB, Mochalova AA, Lokhmatova IA, Manashova MG, Petrenko OV. Nonspecific manifestations of helminthiases in children. Zdorov'e Rebenka. 2015;(8):45-50. (In Russ.)
7. Loboda VF, Glushko KT. The role of sanitary-hygienic education in the development of chronic digestive system pathology in children. Zhurn Grodn Gos Med Un-ta. 2013;(3):43-5. (In Russ.)
8. Ermolenko AE. Etiological classification of tumors and carcinogenesis mechanisms [Elektronnyi resurs]. Mat Morfologiia Elektron Mat Mediko-biol Zhurn. 2012;11(vyp 2). Rezhim dostupa: Data dostupa: 19.08.2019. (In Russ.)
9. Anichkov NM. The doctrine of apoptosis at the present stage. Uch Zap SPbGMU im akad IP Pavlova. 1999;(vyp 4):31-40. (In Russ.)
10. Butel JS. Viral carcinogenesis: revelation of molecular mechanisms and etiology of human disease. Carcinogenesis. 2000 Mar;21(3):405-26.
11. Nadyrov EA, Rogov YuI, Dubrovskiy ACh, Voropaev EV, Achinovich SL, Krylov AYu, i dr. Immunohistochemical methods for the study of neoplasms of various origins: instruktsiia po primeneniiu № 160-1110: utv M-vom zdravookhraneniia Resp Belarus' 11.02.2011 g. Gomel', RB; 2011. 24 р. (In Russ.)
12. Bekish VYa. Method for producing invasive Ascaris eggs. V: Piatyi Respublikanskii s"ezd spetsialistov laboratornoi diagnostiki Belarusi: materialy s"ezda. Minsk, RB; 1997. Р. 140-1. (In Russ.)
13. Sapozhnikov AG, Dorosevich AE. Histological and microscopic technique: rukovodstvo. Smolensk, RF: SAU; 2000. 480 р. (In Russ.)
14. Honeycutt J, Hammam O, Fu CL, Hsieh MH. Controversies and challenges in research on urogenital schistosomiasis-associated bladder cancer. Trends Parasitol. 2014 Jul;30(7):324-32. doi:
15. Ploeg M, Aben KК, Kiemeney LA. The present and future burden of urinary bladder cancer in the world. World J Urol. 2009 Jun;27(3):289-93. doi:
16. Salem HK, Mahfouz S. Changing patterns (age, incidence, and pathologic types) of schistosoma-associated bladder cancer in Egypt in the past decade. Urology. 2012 Feb;79(2):379-83. doi:
17. Shokeir AA. Squamous cell carcinoma of the bladder: pathology, diagnosis and treatment. BJU Int. 2004 Jan;93(2):216-20.
18. Višnjar T, Kocbek P, Kreft ME. Hyperplasia as a mechanism for rapid resealing urothelial injuries and maintaining high transepithelial resistance. Histochem Cell Biol. 2012 Feb;137(2):177-86. doi:
19. Buisson Y. Control of Opisthorchis viverrini infection for cholangiocarcinoma prevention. Bull Soc Pathol Exot. 2017 Feb;110(1):61-67. doi:
20. Smout MJ, Sripa B, Laha T, Mulvenna J, Gasser RB, Young ND, et al. Infection with the carcinogenic human liver fluke, Opisthorchis viverrini. Mol Biosyst. 2011 May;7(5):1367-75. doi:
21. Bekish O-YaL, Bekish VlYa. Mutagenic effect of metabolites of migratory Ascaris larvae (Ascaris suum). Vestsі Nats Akad Navuk Belarusi Ser Biial Navuk. 2000;(2):109-13. (In Russ.)
22. Zorina VV, Bekish O-YaL. The effect of migratory Ascaris larvae on the host genome during pregnancy. Vestn VGMU. 2009;8(2):120-7. (In Russ.)
23. Stibel' VV, Danko NN, Svarchevskiy OA. The effect of intravital secretions of nematodes on the genome of white rats. Teoriia Praktika Parazitar Boleznei Zhivotnykh. 2010;(11):463-6. (In Russ.)
24. Blaszkowsk, J. Disturbance of mouse pregnancy after injection of Ascaris homogenate during early organogenesis. Wiad Parazytol. 2000;46(3):369-78.

Information about authors:
Pabiarzhyn V.V. – Candidate of Biological Sciences, associate professor, doctoral candidate of the Chair of Infectious Diseases, Vitebsk State Order of Peoples’ Friendship Medical University.

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Infectious Diseases. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Vyacheslav V. Pobyarzhin.

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