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Kazeko L.А., Zakharava V.А., Anfinogenova E.А., Cherstvoy E.D.
The peculiarities and prognostic value of the matrix metalloproteinase 8 expression character in periodontal pathology
Belarusian State Medical University, Minsk, Republic of Belarus

Vestnik VGMU. 2019;18(5):99-106.

Objectives. To establish the significance of matrix metalloproteinase 8 (MMP8) for the diagnosis and prognosis of the periodontal pathology in patients with periodontitis at the disease manifestation stage using morphometric analysis of the MMP8 expression in biopsy material.
Material and methods. A gingival biopsy material from 156 patients was analysed: control group (gingivitis only, n=7), rapidly progressive (n=64), chronic simple (n=23) and chronic complex (n=62) periodontitis. The data were processed using the AperioImageScopev 9.0 and Statistica 10.0, р<0.05.
Results. The expression of the MMP8 occurred in all samples in the form of cytoplasmic staining of the inflammatory infiltrate cells and fibroblasts. The correlation of the MMP8 stromal expression (positivity – ρ=0.59 and intensity – ρ=0.52) with the epithelial component damage in the process of inflammation was revealed. The highest variability of the MMP8 expression («hot points» analysis) was in the group of patients with rapidly progressive periodontitis (up to 83%), decreased up to 63% and 54% in the groups of control and chronic complex periodontitis, respectively, and did not exceed 20% in the group of chronic simple periodontitis. At the same time, complex analysis of biopsy material in 4-6 random fields of view in each case showed the highest positivity and intensity of the MMP8 expression in the group of patients with the signs of gingivitis, with the decrease in the groups of chronic simple and rapidly progressive periodontitis and the lowest parameters in the group of chronic complex periodontitis.
Conclusions. For the prognosis and differential diagnosis of rapidly progressive periodontitis in comparison with chronic simple and chronic complex periodontitis at the disease manifestation stage the intensity and positivity of the MMP8 expression can be considered to be the most informative indicators .
Key words: periodontitis, matrix metalloproteinase 8, immunohistochemistry, expression, prognosis.

The research work was conducted within the frames of the project «To elaborate and introduce into practice the method of diagnosing of rapidly progressive periodontitis on the basis of the determination of matrix metalloproteinases in the periodontium tissues», the subprogram «Internal medicine» of the SSTP «New technologies of diagnosing, treatment and prevention», SR №20180436.

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Information about authors:
Kazeko L.А. – Candidate of Medical Sciences, associate professor, head of the Chair of Restorative Dentistry No.1, Belarusian State Medical University,
Zakharava V.А. – Candidate of Medical Sciences, associate professor of the Chair of Pathologic Anatomy, Belarusian State Medical University,
Anfinogenova E.А. – Candidate of Medical Sciences, associate professor of the Chair of Pathologic Anatomy, Belarusian State Medical University,
Cherstvoy E.D. – Doctor of Medical Sciences, professor of the Chair of Pathologic Anatomy, Belarusian State Medical University,

Correspondence address: Republic of Belarus, 220116, Minsk, 83 Dzerzhinskogo ave., Belarusian State Medical University, Chair of Restorative Dentistry No.1. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. –Lyudmila А. Kazeko.

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