Menu

A+ A A-

Download article

DOI: https://doi.org/10.22263/2312-4156.2019.5.99

Kazeko L.А., Zakharava V.А., Anfinogenova E.А., Cherstvoy E.D.
The peculiarities and prognostic value of the matrix metalloproteinase 8 expression character in periodontal pathology
Belarusian State Medical University, Minsk, Republic of Belarus

Vestnik VGMU. 2019;18(5):99-106.

Abstract.
Objectives. To establish the significance of matrix metalloproteinase 8 (MMP8) for the diagnosis and prognosis of the periodontal pathology in patients with periodontitis at the disease manifestation stage using morphometric analysis of the MMP8 expression in biopsy material.
Material and methods. A gingival biopsy material from 156 patients was analysed: control group (gingivitis only, n=7), rapidly progressive (n=64), chronic simple (n=23) and chronic complex (n=62) periodontitis. The data were processed using the AperioImageScopev 9.0 and Statistica 10.0, р<0.05.
Results. The expression of the MMP8 occurred in all samples in the form of cytoplasmic staining of the inflammatory infiltrate cells and fibroblasts. The correlation of the MMP8 stromal expression (positivity – ρ=0.59 and intensity – ρ=0.52) with the epithelial component damage in the process of inflammation was revealed. The highest variability of the MMP8 expression («hot points» analysis) was in the group of patients with rapidly progressive periodontitis (up to 83%), decreased up to 63% and 54% in the groups of control and chronic complex periodontitis, respectively, and did not exceed 20% in the group of chronic simple periodontitis. At the same time, complex analysis of biopsy material in 4-6 random fields of view in each case showed the highest positivity and intensity of the MMP8 expression in the group of patients with the signs of gingivitis, with the decrease in the groups of chronic simple and rapidly progressive periodontitis and the lowest parameters in the group of chronic complex periodontitis.
Conclusions. For the prognosis and differential diagnosis of rapidly progressive periodontitis in comparison with chronic simple and chronic complex periodontitis at the disease manifestation stage the intensity and positivity of the MMP8 expression can be considered to be the most informative indicators .
Key words: periodontitis, matrix metalloproteinase 8, immunohistochemistry, expression, prognosis.

The research work was conducted within the frames of the project «To elaborate and introduce into practice the method of diagnosing of rapidly progressive periodontitis on the basis of the determination of matrix metalloproteinases in the periodontium tissues», the subprogram «Internal medicine» of the SSTP «New technologies of diagnosing, treatment and prevention», SR №20180436.
     
References

1. Albandar JM. Aggressive periodontitis: case definition and diagnostic criteria. Periodontol 2000. 2014 Jun;65(1):13-26. doi: http://dx.doi.org/10.1111/prd.12014
2. Gupta N, Gupta ND, Gupta A, Khan S, Bansal N. Role of salivary matrix metalloproteinase-8 (MMP-8) in chronic periodontitis diagnosis. Front Med. 2015 Mar;9(1):72-6. doi: http://dx.doi.org/10.1007/s11684-014-0347-x
3. Gupta N, Gupta ND, Goyal L, Moin S, Khan S, Gupta A, et al. The influence of smoking on the levels of matrix metalloproteinase-8 and periodontal parameters in smoker and nonsmoker patients with chronic periodontitis: A clinicobiochemical study. J Oral Biol Craniofac Res. 2016 Nov;6(Suppl 1):S39-S43. doi: http://dx.doi.org/10.1016/j.jobcr.2015.08.004
4. Wahlgren J, Salo T, Teronen O, Luoto H, Sorsa T, Tjäderhane L. Matrix metalloproteinase-8 (MMP-8) in pulpal and periapical inflammation and periapical root-canal exudates. Int Endod J. 2002 Nov;35(11):897-904. doi: http://dx.doi.org/10.1046/j.1365-2591.2002.00587.x
5. Zhang L1, Li X2, Yan H1, Huang L. Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis: a PRISMA-compliant systematic review. Medicine (Baltimore). 2018 Jan;97(3):e9642. doi: http://dx.doi.org/10.1097/MD.0000000000009642
6. Kessenbrock K, Plaks V, Werb Z. Matrix metalloproteinases: regulators of the tumor microenvironment. Cell. 2010 Apr;141(1):52-67. doi: http://dx.doi.org/10.1016/j.cell.2010.03.015
7. Sorsa T, Tjäderhane L, Salo T. Matrix metalloproteinases (MMPs) in oral diseases. Oral Dis. 2004 Nov;10(6):311-8. doi: http://dx.doi.org/10.1111/j.1601-0825.2004.01038.x
8. Sorsa T, Tervahartiala T, Leppilahti J, Hernandez M, Gamonal J, Tuomainen AM, et al. Collagenase-2 (MMP-8) as a point-of-care biomarker in periodontitis and cardiovascular diseases. Therapeutic response to non-antimicrobial properties of tetracycline. Pharmacol Res. 2011 Feb;63(2):108-13. doi: http://dx.doi.org/10.1016/j.phrs.2010.10.005
9. Salminen A, Gursoy UK, Paju S, Hyvärinen K, Mäntylä P, Buhlin K, et al. Salivary biomarkers of bacterial burden, inflammatory response, and tissue destruction in periodontitis. J Clin Periodontol. 2014 May;41(5):442-50. doi: http://dx.doi.org/10.1111/jcpe.12234
10. Rathnayake N, Akerman S, Klinge B, Lundegren N, Jansson H, Tryselius Y, et al. Salivary biomarkers of oral health: a cross-sectional study. J Clin Periodontol. 2013 Feb;40(2):140-7. doi: http://dx.doi.org/10.1111/jcpe.12038
11. Miller CS, King CP Jr, Langub MC, Kryscio RJ, Thomas MV. Salivary biomarkers of existing periodontal disease: a cross-sectional study. J Am Dent Assoc. 2006 Mar;137(3):322-9. doi: http://dx.doi.org/10.14219/jada.archive.2006.0181
12. Kraft-Neumärker M, Lorenz K, Koch R, Hoffmann T, Mäntylä P, Sorsa T, et al. Full-mouth profile of active MMP-8 in periodontitis patients. J Periodontal Res. 2012 Feb;47(1):121-8. doi: http://dx.doi.org/10.1111/j.1600-0765.2011.01416.x
13. Herr AE, Hatch AV, Throckmorton DJ, Tran HM, Brennan JS, Giannobile WV, Singh AK. Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proc Natl Acad Sc. USA. 2007 Mar;104(13):5268-73. doi: http://dx.doi.org/10.1073/pnas.0607254104
14. Gursoy UK, Könönen E, Huumonen S, Tervahartiala T, Pussinen PJ, Suominen AL, et al. Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis. J Clin Periodontol. 2013 Jan;40(1):18-25. doi: http://dx.doi.org/10.1111/jcpe.12020

Information about authors:
Kazeko L.А. – Candidate of Medical Sciences, associate professor, head of the Chair of Restorative Dentistry No.1, Belarusian State Medical University,
ORCID: https://orcid.org/0000-0002-0821-0366
Zakharava V.А. – Candidate of Medical Sciences, associate professor of the Chair of Pathologic Anatomy, Belarusian State Medical University,
ORCID: https://orcid.org/0000-0003-2050-9800
Anfinogenova E.А. – Candidate of Medical Sciences, associate professor of the Chair of Pathologic Anatomy, Belarusian State Medical University,
ORCID: https://orcid.org/0000-0002-2696-1374
Cherstvoy E.D. – Doctor of Medical Sciences, professor of the Chair of Pathologic Anatomy, Belarusian State Medical University,
ORCID: https://orcid.org/0000-0002-2554-1431

Correspondence address: Republic of Belarus, 220116, Minsk, 83 Dzerzhinskogo ave., Belarusian State Medical University, Chair of Restorative Dentistry No.1. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. –Lyudmila А. Kazeko.

Поиск по сайту