A+ A A-

Download article


Zybleva S.V., Zyblev S.L.
The dynamics of CD3+CD4+CD8+ T-lymphocytes in kidney transplant recipients in the early and late post-transplantation period
Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus

Vestnik VGMU. 2020;19(1):73-79.

Objectives. To study the dynamics of the level of CD3+CD4+CD8+ T-lymphocytes on different course variants of the post-transplantation period in patients after kidney transplantation.
Material and methods. The study included 165 recipients who underwent kidney transplantation. Immunological examination was conducted before the transplantation and on the 3rd, 7th, 30th, 90th, 180th and 360th day after the transplantation. Four groups were formed: KTR1 –  patients with satisfactory primary and late graft function, KTR2 –patients with satisfactory primary function and late dysfunction, KTR3 – those with primary dysfunction and satisfactory late function, KTR4 – patients with primary and late graft dysfunction. When creatinine levels were lower than 300 μmol/L on the 7th day, the function was considered as satisfactory primary one. Primary graft dysfunction was considered when creatinine values were equal to or higher than 300 μmol/L and when dialysis was necessary during the first week after transplantation. Satisfactory late graft function was characterized by the creatinine concentration after a year below 150 μmol/L, the absence of episodes of transplant rejection and dialysis during the first year.
Results. In KTR1 and KTR2 groups, there were no significant differences in CD3+CD4+CD8+ for 3 months. In these groups, a significant decrease of CD3+CD4+CD8+ was observed by the 7th day followed by an increase by the 30th day. Beginning with the 180th day, a significant increase in the level of CD3+CD4+CD8+ was observed in the KTR2 group compared to the KTR1 group. A significant prevalence of CD3+CD4+CD8+ was found in the KTR1 group before surgery and up to 3 months compared to the KTR3 and the KTR4 groups. Further, we observed a decrease in T-lymphocytes in the KTR3 and KTR4 groups. On the 180th day there were no significant differences between the KTR3, KTR4 and KTR1groups. One year after transplantation, the levels of CD3+CD4+CD8+ were significantly lower in the KTR1 and KTR3 groups than those in the KTR4 group.
Conclusions. Patients with satisfactory primary graft function are characterized by the decrease in the level of CD3+CD4+CD8+, which is not observed in the groups with primary graft dysfunction. Graft dysfunction in the late post-transplantation period is characterized by the increase in the CD3+CD4+CD8+ level compared to groups with satisfactory late graft function.
Key words: kidney transplantation, CD3+CD4+CD8+, renal graft dysfunction.


1. Gao JF, McIntyre MS, Juvet SC, Diao J, Li X, Vanama RB, et al. Regulation of antigen-expressing dendritic cells by double negative regulatory T cells. Eur J Immunol. 2011 Sep;41(9):2699-708. doi:
2. Sakaguchi S, Miyara M, Costantino CM, Hafler DA. FOXP3+ regulatory T cells in the human immune system. Nat Rev Immunol. 2010 Jul;10(7):490-500. doi:
3. Balatskaya NV, Eremeeva EA, Slepova OS, Ryabina MV, Kulikova IG, Sorozhkina ES. The subpopulation of peripheral blood lymphocytes in patients with age-related macular degeneration. Med Immunologiia. 2015;17(5):461-6. (In Russ.)
4. Bunce C, Wormald R. Causes of blind certifications in England and Wales: april 1999 – march 2000. Eye. 2008;22;905-11.
5. Nascimbeni M, Shin EC, Chiriboga L, Kleiner DE, Rehermann B. Peripheral CD4(+)CD8(+) T cells are differentiated effector memory cells with antiviral functions. Blood. 2004 Jul;104(2):478-86 doi:
6. Khaydukov SV. Small subpopulations of T helper cells (Th naive thymic, Th naive central, TH9, TH22 ia CD4 CD8 twice positive T cells). Med Immunologiia. 2013;15(6):503-12. (In Russ.)
7. Iwatani Y, Hidaka Y, Matsuzuka F, Kuma K, Amino N. Intrathyroidal lymphocyte subsets, including unusual CD4+CD8+ cells and CD3loTCR alpha-beta loy-CD4-CD8- cells, in autoimmune thyroid disease. Clin Exp Immunol. 1993 Sep;93(3):430-6.
8. Bang K, Lund M, Wu K, Mogensen SC, Thestrup-Pedersen K. CD4+CD8+ (thymocyte-like) T lymphocytes present in blood and skin from patients with atopic dermatitis suggest immune dysregulation. Br J Dermatol. 2001 Jun;144(6):1140-7.
9. Choi YJ, Park HJ, Park HJ, Jung KC, Lee JI. CD4hiCD8low double-positive t cells are associated with graft rejection in a nonhuman primate model of islet transplantation. J Immunol Res. 2018 Jul;2018:3861079. doi:
10. Cantaluppi V, Dellepiane S, Tamagnone M, Medica D, Figliolini F, Messina M, et al. Neutrophil gelatinase associated lipocalin is an early and accurate biomarker of graft function and tissue regeneration in kidney transplantation from extended criteria donors. PLoS One. 2015 Jun;10(6):e0129279. doi:
11. Massart A, Ghisdal L, Abramowicz M, Abramowicz D. Operational tolerance in kidney transplantation and associated biomarkers. Clin Exp Immunol. 2017 Aug;189(2):138-157. doi:

Information about authors:
Zybleva S.V. – Candidate of Medical Sciences, academic secretary, immunologist, Republican Research Center for Radiation Medicine and Human Ecology;
Zyblev S.L. – Candidate of Medical Sciences, associate professor, surgeon of the surgical department (transplantation, reconstructive and endocrine surgery), Republican Research Center for Radiation Medicine and Human Ecology,

Correspondence address: Republic of Belarus, 246000, Gomel, 5 Ilicha str., Republican Research Center for Radiation Medicine and Human Ecology, Scientific Department. E-mail: zyb-svetlana@ – Svetlana V. Zybleva.

Поиск по сайту