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DOI: https://doi.org/10.22263/2312-4156.2020.2.28

Tkachenko A.S.
The influence of E407a food additive on granulocyte apoptosis intensity in an experiment in vitro
Kharkiv National Medical University, Kharkiv, Ukraine

Vestnik VGMU. 2020;19(2):28-34.

Abstract.
Objectives. To analyze the content of active caspase-3 in peripheric blood granulocytes of rats exposed to short-term high concentrations of E407a food additive for the assessment of apoptosis intensity.
Material and methods. The experiment was performed using 8 female WAG rats whose blood was incubated with semi-refined carrageenan (E407a) solution at the final concentration of 8 g/L during 1 and 2 hours. The level of active caspase-3 in granulocytes was determined by flow cytometry using antibodies to active caspase-3 labelled with Alexa Fluor® 647 fluorochrome. The amount of cells with active caspase-3 and mean fluorescence intensity (MFI) in caspase-3+ granulocytes were evaluated after gating the corresponding region. The statistical data processing was performed by Kruskal-Wallis and post-hoc Dunn’s tests.
Results. It has been shown that the short-term incubation of experimental animals blood with E407a food additive affects neither the content of active caspase-3 in blood granulocytes nor MFI. Thus, semi-refined carrageenan is not capable of inducing granulocyte apoptosis in an experiment in vitro.
Conclusions. Direct exposure of granulocytes to high concentrations of E407a food additive does not promote their apoptosis.
Key words: food additives, carrageenan, Е407а, apoptosis, active caspase-3.

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Information about authors:
Tkachenko A.S. – Candidate of Medical Sciences, associate professor of the Chair of Biochemistry, Kharkiv National Medical University,
ORCID: https://orcid.org/0000-0002-1029-1636.

Correspondence address: Ukraine, 61022, Kharkiv, 4 Nauky ave., Kharkiv National Medical University, Chair of Biochemistry. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anton S. Tkachenko.

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