DOI: https://doi.org/10.22263/2312-4156.2020.4.22
Belyaeva L.Eu., Ligetskaya I.V., Pauliukevich A.N., Lazuko S.S., Ladik Yu.S.
The gender-related peculiarities of ATP-sensitive potassium channels activity in the prenatally stressed rats’ coronary arteries
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(4):22-29.
Abstract.
Objectives. To investigate the role of ATP-sensitive potassium channels (KATP-channels) in the coronary arteries tone regulation in 5-months-old male and female rats whose mothers were exposed to chronic unpredictable stress during their pregnancy.
Material and methods. Primigravida mongrel 4-months-old rats weighing 220-240 g (n=16) were divided into the «Control» and «Stress» groups. Rats from the «Stress» group were exposed to chronic unpredictable stress (one-day fasting, twenty-minute immobilization in water and one-day contact with cats’ excrements) repeatedly from the 2nd to the 16th pregnancy days at different intervals. After birth, rats’ offspring were kept in standard vivarium conditions and at the age of 5 months they were anesthetized with nembutal (60 mg/kg, i.p.). On the isolated by Langendorff method isotonically contracted heart (n=49) perfused with Krebs-Henseleight solution at the coronary flow velocity (CFV) of 6, 8 and 10 ml/min coronary perfusion pressure (CPP) was registered. When CVF was 10 ml/min glibenclamide («Sigma», USA), PotATP-channels blocker, was infused into the perfusion solution (10 micromol/l). The percentage of the CPP increment after glibenclamide infusion indicated the activity of KATP-channels in the coronary arteries.
Results. In isolated hearts from prenatally stressed female rats the CPP at CFV of 10 ml/min was by 25.3% lower than that in isolated hearts from the control female rats’ offspring, whereas the CPP in isolated hearts from prenatally stressed males did not differ from the control values. The CPP increment after glibenclamide infusion into the perfusion solution in isolated hearts from prenatally stressed female rats was approximately 2 times higher than that in the hearts of control female offspring (p=0.007). In contrast to these results, the glibenclamide-induced CPP increment in isolated hearts from prenatally stressed male rats was by 26.3% lower compared with control male offspring (p=0.024).
Conclusions. It has been shown for the first time that the impact of chronic unpredictable stress on pregnant rats produces an enhancement of KATP-channels activity in the coronary arteries cells of their 5-months-old female offspring and the reduction of their activity in the coronary arteries of male offspring.
Key words: prenatal stress, coronary arteries tone, KATP-channels.
The authors express their gratitude to the anonymous reviewers for their careful perusal of this paper and the remarks made.
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Information about authors:
Belyaeva L.Eu. – Candidate of Medical Sciences, associate professor, head of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Ligetskaya I.V. – Master of Biological Sciences, senior lecturer of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Pauliukevich A.N. – Master of Medical Sciences, senior lecturer of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Lazuko S.S. – Candidate of Biological Sciences, associate professor, head of the Chair of Normal Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Ladik Yu.S. – the fifth-year medical student, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of of Pathologic Physiology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Lyudmila Eu. Belyaeva.
