DOI: https://doi.org/10.22263/2312-4156.2020.5.59
Fedzianin S.D., Okulich V.K.
The monitoring of the resistance of gram-negative microflora, isolated in the patients with purulent wounds
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(5):59-65.
Abstract.
Objectives. To study the changes in the resistance of gram-negative flora to antibacterial drugs in patients with purulent wounds.
Material and methods. On the basis of the bacteriological laboratory, in 2006-2007 and in 2019-2020, 92 and 99 patients with purulent wounds of various localizations, respectively, were examined by bacteriological methods. These patients underwent hospital treatment at the department of purulent surgery of Vitebsk Regional Clinical Hospital.
The microorganisms species belonging was determined automatically on the ATB Expression bioMerieux biochemistry analyzer using test-systems: ID 32 E − for enterobacteria, ID 32 GN − for gram-negative flora (France) and ID-ENT test-systems for enterobacteria (BelVitunipharm), that were developed by us.
The resistance of microorganisms was determined automatically on the ATB Expression bioMerieux biochemistry analyzer with the use of test-systems: ATB PSE − for pseudomonads, rapid ATB E − for enterobacteria (France) and the AB-PSEV, AB-ENTER, AB-GRAM test-systems, developed by us for pseudomonads, enterobacteria and gram-negative flora (BelVitunipharm).
Results. It has been found, that in the etiological structure of purulent wounds the proportion of K.pneumoniae increased by 12.25% (p<0.05), and that of A.baumannii increased by 14. 69% (p<0.05). For the time of monitoring, the resistance of gram-negative flora to almost all of the antibacterial drugs has significantly increased. Only colistin and tigecycline retained high antimicrobic activity.
Conclusions. Over 13 years, significant changes have occurred in the etiological structure of gram-negative microorganisms isolated in the patients with purulent wounds. A reliable increase in the proportion of gram-negative problematic pathogens, such as K.pneumoniae and A.baumannii, has been noted. During the period of monitoring, the resistance of gram-negative flora to almost all of the antibacterial drugs has significantly increased. The results obtained should be taken into account, while employing antibiotic therapy in patients with purulent wounds.
Key words: resistance, purulent wound, enterobacteria, pseudomonads, klebsiella, acinetobacter.
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Information about authors:
Fedzianin S.D. – Candidate of Medical Sciences, associate professor of the Chair of Hospital Surgery with the courses of Urology & Pediatric Surgery, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0003-4771-4724
Okulich V.K. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-8226-6405
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Hospital Surgery with the courses of Urology & Pediatric Surgery. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Siarhei D. Fedzianin.
