Microbiome of the uterine cavity in female patients with chronic endometritis
Gomel State Medical University, Gomel, Republic of Belarus
Vestnik VGMU. 2020;19(6):79-85.
Objectives. To study the microbiome of the uterine cavity in female patients with chronic endometritis and patients in the comparison group using the 16srRNA sequencing method.
Material and methods. 128 patients of the reproductive age were examined. The study group included 91 (71.09%) patients with immunohistochemical signs of chronic endometritis, the comparison group was composed of 37 (28.91%) women with normal endometrium. The microbiome of the uterine cavity was investigated with the help of the 16srRNA sequencing method.
Results. By sequencing the 16srRNA gene regions, the genetic material of microorganisms in the uterine cavity was determined in 34 (91.89%) patients without chronic endometritis and in 82 (90.11%) patients of the study group (p=0.72). In the majority of patients in the comparison group, one type of microorganisms was isolated (χ2=29.11, p<0.001), the combination of four or more microorganisms was identified in 45 (52.75%) patients of the study group, (χ2=21.21, p<0.001). Corynebacterium spp., Dialister spp., Eubacterium spp., Bacteroides spp., Leptotrichia spp., Porphyromonas spp. were found statistically significantly more often in the microbiome of the uterine cavity in patients with chronic endometritis. Most often, Prevotella spp. was detected in female patients of the study group – in 23 (25.27%) patients, in the comparison group this microorganism was isolated in 1 (2.70%) woman (χ2=7.83, p=0.006).
The microbiome of the uterine cavity in 75 (82.42%) women with chronic endometritis and in 13 (35.14%) patients of the comparison group contained microorganisms not detected by PCR in the cervical canal (χ2=30.33, p<0.001).
Conclusions. The microbiome of the uterine cavity in patients with chronic endometritis is represented by a wide variety of types of microorganisms and their combinations, forming a unique biocenosis in the uterine cavity, that can be determined by means of the 16srRNA sequencing method.
Key words: chronic endometritis, 16srRNA sequencing, microbiome, biocenosis, Corynebacterium spp., Dialister spp., Eubacterium spp., Bacteroides spp., Leptotrichia spp., Porphyromonas spp., Prevotella spp.
Financing. The study was sponsored by the Innovation Fund of the Gomel Regional Executive Committee (State Registration No. 20164462 of 05.12.2016).
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Information about authors:
Lyzikova Yu.A. – Candidate of Medical Sciences, associate professor of the Chair of Obstetrics & Gynecology with the course of the Faculty for Advanced Training & Retraining, Gomel State Medical University,