Menu

A+ A A-

Полный текст статьи

DOI: https://doi.org/10.22263/2312-4156.2024.3.28

A.F. Raduta
The development of dermal-cartilaginous autografts on the surface of a full-thickness skin defect in rats
Republican Research Unitary Enterprise «Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus», Grodno, Republic of Belarus

Vestnik VGMU. 2024;23(3):28-36.

Abstract.
Objectives. To reveal in dynamics the regularities of engraftment and existence on the receptive field of rats skin-cartilaginous autografts obtained from their auricles.
Material and methods. In male Wistar rats, a full-thickness skin defect of 1.2-1.4 cm2 was created in the interscapular region, which was limited by a plastic security fixation. The animals were cut off each ear, from the skin of the inner surface of which a square site of 1 cm2 was cut out. It was divided into 9 pieces and distributed on the wound surface. Macroscopic, histological and electron microscopic methods were used to study the engraftment of dermal-cartilaginous autografts.
Results. In 5 days after transplantation on those parts of the autografts where the cartilaginous support was intact, the chondrocyte layer looked almost the same as in the intact auricle structure. On the sites with the damaged lower chondrocytic cell layer, chondrocyte proliferation was observed in 5-10 days. After 30 days, a drop of fat was present in the cytoplasm of many newly formed chondrocytes, which was observed after 100 and 200 days. Autografts after 400 and 600 days remained as organized as autografts after 100 and 200 days.
Conclusions. In heterotopic autotopic autotransplantation of skin-cartilage grafts, cartilage tissue takes root on the receptive bed and remains viable in a size close to the original one for more than 1.5 years. The skin of the inner surface of the auricle, that was part of the autografts, significantly blocked the contraction of the receptive field.
Keywords: skin-cartilage autografts, full-thickness skin defect, receptive field, auricle, chondrocytes.

Acknowledgments. The author expresses her gratitude to Doctor of Medical Sciences, Prof. Alexander Ostrovsky for his valuable advice and recommendations for the design of the article.

References

1.    Bulgin DV, Kovtun AL, Reshetov IV, Radomskaya EYu. Prospects of creating artificial human tissues and organs based on the method of three-dimensional bioprinting. Vestn Transplantologii Iskusstv Organov. 2023;25(2):63-81. (In Russ.). doi: http://dx.doi.org/10.15825/1995-1191-2023-2-63-81
2.    Onderková A, Kalaskar DM. 3D bioprinting for auricular reconstruction: A review and future perspectives. Int J Bioprinting. 2023;9(6):273-307. doi: http://dx.doi.org/10.36922/ijb.0898
3.    Jang CH, Koo YW, Kim GH. ASC/chondrocyte-laden alginate hydrogel/PCL hybrid scaffold fabricated using 3D printing for auricle regeneration. Carbohydr Polym. 2020 Nov:248:116776. doi: http://dx.doi.org/10.1016/j.carbpol.2020.116776
4.    Can IH, Atilla P, Cakar AN, Onerci M. An animal study on cartilage healing using auricular cartilage as a model. Eur Arch Otorhinolaryngol. 2008 Mar;265(3):307-11. doi: http://dx.doi.org/10.1007/s00405-007-0455-1
5.    Saifzadeh S, Rezazadeh Gh, Ghasemi SM. Role of pinna cartilage autograft on long bone healing in dog: radiographic and biomechanical studies. J Veterinary Res. 2007;61(4):415-21.
6.    Raduta AF, Belanovskaya AB, Shatrova VA, Zaloga IV, Ostrovskiy AA. Features of the structure of the auricle of laboratory rats and coniferous mice. Vesn GrDU іm Ya Kupaly. Ser 5, Ekanomіka Satsyyalogіya Bіyalogіya. 2023;13(1):138-49. (In Russ.)
7.    Bakunovich AA, Yaroshenko YuU, Raduta AF, Konanov EV, Buko VYu, Ostrovskiy AA. Experimental model for assessing the readiness of the wound surface to accept skin grafts. Izv Nats Akad Nauk Belarusi Ser Med Nauk. 2021;18(3):340-50. (In Russ.). doi: http://dx.doi.org/10.29235/1814-6023-2021-18-3-340-350
8.    Bakunovіch AA. A model for evaluating the influence of various factors on the performance of skin grafts. Novosti Mediko-biol Nauk. 2021;21(2):11-7.
9.    Raduta AF, Ostrovskaya AB. Cambial elements of rat auricular cartilage. V: GrGU im Yanki Kupaly; Yanchurevich OV, Ryzhaya AV, redkol. Zoologicheskie chteniya: sb nauch st, posvyashch 125-letiyu d-ra biol nauk Ivana Nikolaevicha Serzhanina. Grodno, RB: ГрГУ; 2023. Р. 239-41. (In Russ.)
10.    GOST 33215-2014. Guidelines for the maintenance and care of laboratory animals. Rules of equipment of premises and organization of procedures. Vved. 2016-07-01. Izd ofits. Moscow, RF: Standartinform; 2016. VI, 19 р. (In Russ.)
11.    GOST 33216-2014. Guidelines for the housing and care of laboratory animals. Rules for the housing and care of laboratory rodents and rabbits. Vved 2016-07-01. Izd ofits. Moscow, RF: Standartinform; 2016. VI, 16 р. (In Russ.)
12.    Good laboratory practice = Nalezhnaya labaratornaya praktyka: TKP 125-2008 (02040). Vved 2008-03-28. Izd ofits. Minsk, RB: M-vo zdravookhraneniya Resp Belarus’; [2008]. IV, 39 р. (In Russ.)
13.    Reynolds ES. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17(1):208-12. doi: http://dx.doi.org/10.1083/jcb.17.1.208
14.    Raduta AF. Closure of penetrating holes in the auricles of laboratory rats and hamsters. V: Trushel’ NA, red. Aktual’nye problemy morfologii na sovremennom etape [Electronic resource]: sb nauch st, posvyashch 85-letiyu kmn, dots SP Yaroshevicha UO «Belorusskii gosudarstvennyi meditsinskii universitet», Minsk, Resp Belarus’, okt 2023 g. Minsk, RB: BGMU; 2023. 1 elektron opt disk (CD-ROM). Р. 409-13. (In Russ.)
15.    Brewer CM, Nelson BR, Wakenight P, Collins SJ, Okamura DM, Dong XR, et al. Adaptations in Hippo-Yap signaling and myofibroblast fate underlie scar-free ear appendage wound healing in spiny mice. Dev Cell. 2021 Oct;56(19):2722-40. doi: http://dx.doi.org/10.1016/j.devcel.2021.09.008
16.    Seifert AW, Muneoka K. The blastema and epimorphic regeneration in mammals. Dev Biol. 2018 Jan;433(2):190-199. doi: http://dx.doi.org/10.1016/j.ydbio.2017.08.007
         

Submitted 17.05.2024
Accepted 14.06.2024

Information about authors:
A.F. Raduta – scientific secretary, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0000-0001-8020-1838,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Alena F. Raduta.      

Поиск по сайту