DOI: https://doi.org/10.22263/2312-4156.2024.3.28
A.F. Raduta
The development of dermal-cartilaginous autografts on the surface of a full-thickness skin defect in rats
Republican Research Unitary Enterprise «Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus», Grodno, Republic of Belarus
Vestnik VGMU. 2024;23(3):28-36.
Abstract.
Objectives. To reveal in dynamics the regularities of engraftment and existence on the receptive field of rats skin-cartilaginous autografts obtained from their auricles.
Material and methods. In male Wistar rats, a full-thickness skin defect of 1.2-1.4 cm2 was created in the interscapular region, which was limited by a plastic security fixation. The animals were cut off each ear, from the skin of the inner surface of which a square site of 1 cm2 was cut out. It was divided into 9 pieces and distributed on the wound surface. Macroscopic, histological and electron microscopic methods were used to study the engraftment of dermal-cartilaginous autografts.
Results. In 5 days after transplantation on those parts of the autografts where the cartilaginous support was intact, the chondrocyte layer looked almost the same as in the intact auricle structure. On the sites with the damaged lower chondrocytic cell layer, chondrocyte proliferation was observed in 5-10 days. After 30 days, a drop of fat was present in the cytoplasm of many newly formed chondrocytes, which was observed after 100 and 200 days. Autografts after 400 and 600 days remained as organized as autografts after 100 and 200 days.
Conclusions. In heterotopic autotopic autotransplantation of skin-cartilage grafts, cartilage tissue takes root on the receptive bed and remains viable in a size close to the original one for more than 1.5 years. The skin of the inner surface of the auricle, that was part of the autografts, significantly blocked the contraction of the receptive field.
Keywords: skin-cartilage autografts, full-thickness skin defect, receptive field, auricle, chondrocytes.
Acknowledgments. The author expresses her gratitude to Doctor of Medical Sciences, Prof. Alexander Ostrovsky for his valuable advice and recommendations for the design of the article.
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Submitted 17.05.2024
Accepted 14.06.2024
Information about authors:
A.F. Raduta – scientific secretary, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0000-0001-8020-1838,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Alena F. Raduta.