Nikolayeva I.V., Sheybak V.M., Lelevich S.V., Kravchuk R.I.
The structure of microbiocenosis of rats’ intestine after acetaminophen administration
Abstract.
Objectives. To evaluate microbiological changes in the intestine on administration of acetaminophen hepatotoxic doses to rats.
Material and methods. Experiments were performed on 24 white male rats weighing 180-200 g. The rats had standard vivarium diet: group 1 – the control group (n=12) – got 2% of starch mucilage intragastrically, group 2 – got intragastrically acetaminophen (Sigma) at the dose of 1500 mg per 1 kg of body weight in the form of 2% solution of starch mucilage 5 times every other day. Hepatotoxicity of the administered acetaminophen dose was evaluated by histological changes in the liver, as well as by the activity of marker enzymes and total bilirubin content. For microbiological examination faeces samples were taken, in which the composition of main representatives of the intestinal microflora: bifidobacteria, lactobacilli, escherichia, opportunistic enterobacteria was determined.
Results. It has been found out that the administration of acetaminophen in hepatotoxic dose leads to the development of dysbiosis, characterized by quantitative and qualitative changes in the intestinal microflora. Intragastric administration of acetaminophen to animals significantly alters the normal flora of the large intestine, reducing the content of anaerobic bacteria, lactobacilli, bifidobacteria and increases populations of opportunistic microorganisms, mostly of aerobic microflora.
The data obtained can be used for further substantiation of the feasibility to develop agents for prevention of negative effects of anti-inflammatory non-steroidal drugs.
Key words: acetaminophen, microbiocenosis, intestine, rats.
