Gorodetskaya I.V., Gusakova E.A.
The effect of iodine-containing thyroid hormones on the intensity of lipid peroxidation in the liver and blood of rats under stress
Аbstract.
In the experiments on 182 mongrel white male rats weighing 220–250 g the influence of iodine-containing thyroid hormones on the stress-induced changes in the intensity of lipid peroxidation has been proved. Stress was modelled by the method of «free swimming in a cage» during one hour. Animals were placed in standard plastic cages (5 animals in each) filled with water up to the level of 15 cm and closed with a net. For the experiment the rats were taken at different stages of stress reaction: at the alarm stage (an hour after stress), at the resistance stage (48 hours after stress), at the exhaustion stage (stress during one hour within 10 days). The intensity of lipid peroxidation in the liver and blood was evaluated according to the concentration of diene conjugates (in nmol/mg of lipids) and malondialdehyde (in nmol/mg of protein). In the course of the experiment it has been established that the alarm stage of stress reaction is accompanied by the activation of lipid peroxidation in the blood, and especially in the liver; the resistance stage is characterized by the tendency to leveling the intensity of this process; at the exhaustion stage the greatest stimulation of lipid peroxidation is observed. The introduction of mercazolil (intragastrically in the doses of 25 mg/kg during 20 days) per se reducing lipid peroxidation intensity in the liver and blood stimulates its greater activation at the alarm- and exhaustion stages and eliminates the normalization of this process at the resistance stage of stress reaction. The injection of L-thyroxin in small, similar to physiological doses (1,5–3,0 tg/kg intragastrically during 28 days) by itself does not affect the intensity of lipid peroxidation in the liver and blood, provides full compensation of this process at the resistance stage, and at the alarm- and exhaustion stages it minimizes the activation of lipid peroxidation.
Key words: iodine-containing thyroid hormones, lipid peroxidation, stress.
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