Frolova A.V., Kosinets A.N., Burak I.I., Denisenko V.L.
New approach to the prevention of exogenous wound infecting
Abstract.
We conducted the analysis of the dependence between inoculated microflora from the air and etiological structure of pyo-inflammatory processes in patients from the surgical department. The sensitivity of isolated from the air and wound contents of the strains of S. aureus to antibiotics and antiseptics has been studied. The comparative study of effective air disinfection in the surgical department by traditional mode and with the help of recirculator «Vityaz», equipped with 2 ozone-free mercury lamps PHILIPS of the type TUV 25W with output of beams with e<200 nm has been made. The correlation between the microbial scenery of the air and species composition of the microflora, inoculated from the wound contents of the patients treated at the department has been revealed. It has been found that despite the variety of species composition of wound infection (52,13%) the dominant role in its appearance and development is played by the gram-positive microflora. The characteristic feature of the microbial pattern of the air in the dressing room when the dressings are done in the presence of additional medical staff and students is the revealing of yeastlike fungi in addition to gram-positive and gram-negative microorganisms. Microbiological studies have confirmed that the developed antiseptics are characterized by antimicrobial activity against not only S. aureus, but also other causative agents of wound infection, and their use for prophylactic and therapeutic purposes enables the reduction of the incidence of the development of pyo-inflammatory diseases and postoperative complications in surgical departments. The introduction into surgical practice of safe air decontamination method with the use of domestic bactericidal ultraviolet wall recirculator «Vityaz» helps to reduce the total microbial contamination of the air 13 times more effectively than the traditional mode of UV exposure.
Key words: air, surgery, recirculator, antiseptics.
References
1. Belozer AA, Smirnov OA, Petkova VA. Infektsionnyi kontrol' za vnutribol'nichnymi infektsiiami v statsionare skoroi meditsinskoi pomoshchi [Infection control of nosocomial infections in the hospital ambulance]. V: Sovremennye problemy epidemiologii, diagnostiki i profilaktiki vnutribol'nichnykh infektsii: materialy Mezhdunar. nauch.-prakt. konf. Saint-Petersburg, RF; 2008. р. 75-77.
2. Organizatsiia sanitarno-gigienicheskikh i protivoepidemicheskikh meropriiatii po profilaktike gnoino-septicheskikh infektsii v otdeleniiakh (kabinetakh) khirurgicheskogo profilia [Organization of sanitary and anti-epidemic measures for the prevention of septic infections in the departments (offices) surgical]. Minsk, RB; 2005. 27 р.
3. Pokrovskii VI, Semina NA. Vnutribol'nichnye infektsii: problemy i puti resheniia [Nosocomial infections: problems and solutions]. Epidemiologiia i infektsionnye bolezni. 2008;(5):12-4.
4. Adarchenko AA, Gudkova EI, Slabko IN, Lastochkina TM, Simonenko LI. Etiologicheskaia struktura vnutribol'nichnykh gnoino-septicheskikh infektsii i printsipy ikh bakteriologicheskoi diagnostiki [Etiological structure of the intrahospital it is purulent-septic infections and the principles of their bacteriological diagnostics]. Zdravookhranenie. 2003;(10):39-41.
5. Bukharin OV. Mekhanizmy persistentsii bakterial'nykh patogenov [Mechanisms of a persistention of bacterial pathogens]. Vestnik RAMN. 2000;(2):43-9.
6. Gostishchev VK. Obshchaia khirurgiia [General Surgery]. Moscow, RF: GEOTAR-MED; 2002. 680 р.
7. Kurochkina OS, Iashina NN. Rezul'taty issledovaniia mikroflory vozdukha v operatsionnoi chistogo khirurgicheskogo otdeleniia [Results of the study of the microflora of air in operating net surgical department]. V: Sbornik statei po rezul'tatam Mezhdunarodnoi 62-oi itogovoi studencheskoi konferentsii im. N. I. Pirogova, 21-23 apr. 2003 g. Tomsk, RF; 2003. р. 238-240.
8. Pittet D, Simon A, Hugonnet S, Pessoa-Silva CL, Sauvan V, Perneger TV. Hand hygiene among physicians: performance, beliefs, and perceptions. Ann Intern Med. 2004 Jul;141(1):1-8.
9. Pittet D. Clean hands reduce the burden of disease. Lancet. 2005 Jul;366(9481):185-7.
10. Poliak MS. Klinicheskaia znachimost' i metodologiia opredeleniia antibiotikov v biosubstratakh [The clinical significance and the methodology for determining antibiotics in biosubstrates]. Saint-Petersburg, RF; 1998. 21 р.
