Sergeyeva S.P., Breslavich I.D, Erofeyeva L.M., Gultyayev M.M.
Dynamics and prognostic value of CD3CD95 lymphocytes, soluble Fas receptor and Fas ligand levels in peripheric blood after ischemic insult
Objectives. To investigate the dynamics CD3CD95 lymphocytes, soluble Fas receptor (sFas) and soluble Fas ligand (sFasL) levels in peripheric blood of patients after ischemic insult, the correlation between them, as well as the relationship between these levels and the neurologic manifestations severity.
Material and methods. In all 60 patients were studied, 25 of them were healthy controls. By means of ELISA sFas and sFasL plasma levels were detected. Fas protein (CD95) expression on CD3 lymphocytes surfaces was detected using flow cytometry. To objectivize the estimation of the patients’ severity condition, focal neurologic deficiency expressiveness the point scale of stroke assessment of National Institute of Health (NIH) was used.
Results. The level of sFasL and sFasL/sFas ratio correlated with the severity of patients’ condition after ischemic insult and neurologic disorders (p<0,05).
Conclusions. The changes of plasma level of sFasL and sFasL/sFas ratio are prognostic markers and may be used in clinical practice for determining the management of patients with ischemic insult. Fas induced apoptosis mechanisms seem to be a worthwhile target for the search of new ways in diagnosing, assessment of prognosis and treatment efficacy of patients suffering from ischemic insult.
Key words: apoptosis, ischemic insult, lymphocytes.
1. Mathers CD, Boerma T, Ma Fat D. Global and regional causes of death. Br Med Bull. 2009;92:7-32.
2. Morillasa P, Andradea H, Castilloa J, Quilesa J, Bertomeu-Gonzáleza V, Corderoa А, Tarazónb Е, Rosellób Е, Portolésb М, Riverab М, Bertomeu-Martíneza V. Inflammation and Apoptosis in Hypertension. Relevance of the Extent of Target Organ Damage. Rev Esp Cardiol. 2012;65(9):819–825.
3. Saino O, Taguchi A, Nakagomi T, Nakano-Doi A, Kashiwamura S, Doe N, Nakagomi N, Soma T, Yoshikawa H, Stern DM, Okamura H, Matsuyama T. Immunodeficiency reduces neural stem/progenitor cell apoptosis and enhances neurogenesis in the cerebral cortex after stroke. J Neurosci Res. 2010;88(11):2385–97.
4. Sairanen T, Karjalainen-Lindsberg ML, Paetau A. Ijäs P, Lindsberg PJ. Apoptosis dominant in the periinfarct area of human ischaemic stroke-a possible target of antiapoptotic treatments. Brain. 2006;129(1):189–199.
5. Niu FN, Zhang X, Hu XM, Chen J, Chang LL, Li JW, Liu Z, Cao W, Xu Y. Targeted mutation of Fas ligand gene attenuates brain inflammation in experimental stroke. Brain Behav Immun. 2012;26(1):61–71.
6. Strasser A, Jost PJ, Nagata S. The many roles of FAS receptor signaling in the immune system. Immunity. 2009;30(2):180-192.
7. Hoke M, Schillinger M, Zorn G, Wonnerth А, Amighi J, Mlekusch W, Speidl W, Maurer G, Koppensteiner R, Minar Е, Wojta J, Niessner А. The prognostic impact of soluble apoptosis-stimulating fragment on mortality in patients with carotid atherosclerosis. Stroke. 2011;42:2465-2470.
8. Ramos-Fernandez M, Bellolio MF, Stead LG. Matrix metalloproteinase-9 as a marker for acute ischemic stroke: a systematic review. Journal of Stroke and Cerebrovascular Diseases. 2011;20(1):47-54.