Evdokimova O.V., Gorodetskaya I.V.
The effect of iodine-containing thyroid hormones on the synthesis of heat shock proteins in rats’ brain during stress and adaptation
Education establishment "Vitebsk State Order of Peoples’ Friendship Medical University"
Abstract.
Previously the stimulating effect of iodine-containing thyroid hormones (ICTH) on the expression of inducible 70-kDa heat shock proteins (HSPs-70) in the myocardium has been shown, but ICTH effect on the stress-induced synthesis of HSPs in the brain has not been determined. At the same time it has been established that HSPs-70 are the important component of the endogenous protection of the brain from the damage. The purpose of this work is to study the effect of ICTH on the expression of heat shock proteins in the brain during stress. The experiments were conducted on 96 adult white mongrel male rats weighing 220-250 g. It has been shown that physical (the stay of rats in the cold chamber with t 4°C for 30 minutes), chemical (a single 3,5 g/kg intragastric administration of 25% ethanol solution), and emotional (free swimming in the cage for 30 minutes (FSC)) stresses lead to the stimulation of HSPs-70 expression in the brain. Experimental hypothyroidism (intragastric administration of mercazolil dosed 25 mg/kg in 1% starch paste during 20 days) per se inhibits the synthesis of HSPs-70 in the brain tissue and prevents their accumulation under all studied kinds of stress, as well as during the adaptation to stress (the 1st day – FSC during 1 minute, the 2nd day – during 3 minutes, the 3rd day– during 5 minutes). L-thyroxin administered at low doses (intragastrically 1,5-3,0 µg/kg in 1% starch paste during 28 days) by itself stimulates HSPs-70 synthesis and provides their greater accumulation in the brain of rats under physical, chemical and emotional stresses. The stimulation by ICTH of HSPs-70 expression in the brain under stress and during adaptation reveals a new, previously unknown aspect of their anti-stress action.
Key words: iodine-containing thyroid hormones, stress, heat shock proteins.
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