DOI: https://doi.org/10.22263/2312-4156.2016.1.48
Yupatov G.I.*, Pryshchepenka V.A.**, Kornilov A.V.***
Hyaluronidase activity of blood serum in patients with hepatic cirrhosis
*Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University», Vitebsk, Republic of Belarus
**Public Health Establishment «Vitebsk Regional Clinical Maternity Hospital», Vitebsk, Republic of Belarus
***Public Health Establishment «Vitebsk Town Central Clinical Hospital», Vitebsk, Republic of Belarus
Vestnik VGMU. 2016;15(1):48-54.
Abstract.
Objectives. To determine the level of blood serum hyaluronidase activity in patients with hepatic cirrhosis.
Material and methods. Blood serum hyaluronidase activity was defined by the rivanol clot method. Hyaluronidase activity determination is based on the formation of the clot of rivanol with hyaluronic acid which is in an inverse proportion to the depolymerization of the latter under the effect of hyaluronidase activity of various origin. The serum titer, in which the activity was determined, as well as the size of rivanol clot were estimated.
32 patients suffering from cirrhosis of the liver were examined. Severity class of the disease was by Child-Pugh A (n=5), B (n=12), C (n=15). 18 out of these cases (56%) were men and 14 (44%) women. The average age of the studied group made up 54+10,82 years. The control group included 24 donors from the blood transfusion station, 12 out of them (54%) were men and 10 (46%) women. The average age of the control group made up 40,5+10,1 years.
Results. As a result of the study of blood serum hyaluronidase activity of the patients having hepatic cirrhosis it was established that the serum titer in which hyaluronidase activity was determinated in the patients with cirrhosis of the liver made up 1/8000–1/16000 which was 2-4 times higher than the maximum titer of serum in the group of donors (1/4000). It was determined that in patients suffering from hepatic cirrhosis on the 7th day of treatment a decrease of hyaluronidase activity (р<0,05) was observed. At the same time, the level of hyaluronidase activity despite the provided treatment was reliably higher than that in the donors (р<0,05).
Key words: hyaluronidase activity, hepatic cirrhosis.
References
1. Kuznetsov VI, Bozhko AP, Solodkov AP, Gorodetskaya IV, Kuznetsov VI, red; M-vo zdravookhraneniia Resp Belarus', Viteb gos med un-t. Normal'naia fiziologiia [Normal physiology]: kurs lektsii. Vitebsk, RB; 2010. 612 р.
2. M-vo zdravookhraneniia Resp Belarus', Resp nauch-prakt tsentr med tekhnologii, informatizatsii, upravleniia i ekonomiki zdravookhraneniia. Zdravookhranenie v Respublike Belarus' [Health care in Republic of Belarus]: ofits stat sb za 2013 g. Minsk, RB: RNMB; 2014. 280 р.
3. Gao B, Bataller R. Alcoholic liver disease: pathogenesis and new therapeutic targets. Gastroenterology. 2011 Nov;141(5):1572-85.
4. Prishchepenko VA, Prishchepenko OA. Rasprostranennost', zabolevaemost', struktura zabolevaemosti u patsientov s razlichnymi zabolevaniiami pecheni [Prevalence, incidence, structure of incidence at patients with various diseases of a liver]. V: Studencheskaia meditsinskaia nauka XXI veka: materialy XIII mezhdunar nauch-prakt konf, 14-15 noiab 2013 g. Vitebsk, RB: VGMU; 2013. Р. 139-41.
5. Sherlok Sh, Duli Dzh. Zabolevaniia pecheni i zhelchnykh putei [Diseases of a liver and bilious ways]: per s angl. Moscow, RF: GEOTAR-Media; 1999. 864 р.
6. Radchenko VG, Shabrov FV, Zinovyeva EN. Osnovy klinicheskoi gepatologii. Zabolevaniia pecheni i biliarnoi sistemy [Fundamentals of clinical hepatology. Diseases of a liver and biliarny system]: rukovodstvo. Saint Petersburg, RF: Dialekt; Moscow, RF: BINOM; 2005. 864 р.
7. Ivashkin VT, gl red. Klinicheskie rekomendatsii. Gastroenterologiia [Clinical recommendations. Gastroenterology]. 2-e izd ispr i dop. Moscow, RF: GEOTAR-Media; 2009. 207 р.
8. Martynov AI, Mukhin NA, Moiseev VS, Galyavich AS. Vnutrennie bolezni [Internal diseases]: ucheb dlia vuzov: v 2 t. Moscow, RF: GEOTAR-Media; 2004. T 2. 648 р.
9. Pinzani М, Rosselli M, Zuckermann M. Liver cirrhosis. Best Practice & Research Clinical Gastroenterology. 2011 Apr;25(2):281-90.
10. Kamyshnikov VS. Laboratornaia diagnostika vnutrennikh i khirurgicheskikh boleznei [Laboratory diagnosis of internal and surgical diseases]: ucheb posobie dlia slushatelei sistemy dopoln obrazovaniia vzroslykh po med spetsial'nostiam. Minsk, RB: Adukatsyia і vykhavanne; 2012. 583 р.
11. Meyer K. Hyaluronidases. In: Boye PD. Enzimes. New York: Academic Press; 1971. P. 307-20.
12. Zimmermann K, Preinl G, Ludwig H, Greulich KO. Inhibition of hyaluronidase by dextransulfate and its possible application in anticancer treatment. J Cancer Res Clin Oncol. 1983;105(2):189-90.
13. Ivanikov IO, Syutkin VE, Govorun VM. Obshchaia gepatologiia [General hepatology]: ucheb posobie. 2-e izd pererab i dop. Moscow, RF: MAKS-Press; 2002. 111 р.
14. Klinicheskie protokoly diagnostiki i lecheniia bol'nykh s zabolevaniiami organov pishchevareniia [Clinical protocols of diagnostics and treatment of patients with diseases of the digestive system]: pril 6 k prikazu M-va zdravookhraneniia Resp Belarus' ot 19.05.2005 g № 274 [Elektronnyi resurs]. Rezhim dostupa: http://minzdrav.gov.by/ru/static/spavochno-infirm/protololy_lechenia/protokoly_2005. Data dostupa: 11.03.2016.
15. Generalov II, Litvyakov AM, Kunder EV, Moiseeva AM; M-vo zdravookhraneniia Resp Belarus', Viteb gos ordena Druzhby narodov med un-t. Opredelenie fermentativnoi (gialuronidaznoi i dezoksiribonukleaznoi) aktivnosti depolimerizuiushchego deistviia pri autoimmunnoi i infektsionnoi patologii [Definition enzymatic (hyaluronidase and DNase) activities of the depolymerizing action at autoimmune and infectious pathology]: instruktsiia po primeneniiu. Vitebsk, RB; 2007. 11 р.
16. Glants S. Mediko-biologicheskaia statistika [Medicobiological statistics]: per s angl. Moscow, RF: Praktika; 1999. 459 р.
