DOI: https://doi.org/10.22263/2312-4156.2016.1.99
Khishova O.M.*, Dubashynskaya N.V.*, Adamenko Y.Y.**
Production technology and quality assessment of liquid extract of valerian rhizomes and roots
*Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University», Vitebsk, Republic of Belarus
**Gomel Republican Unitary Enterprise «Pharmacy», chemist’s shop N156, Republic of Belarus
Vestnik VGMU. 2016;15(1):99-105.
Abstract.
The purpose of the study was to develop the technology of obtaining a liquid extract of valerian rhizomes and roots at a ratio of 1:1 and to assess its quality.
For extraction of valerian rhizomes and roots ethyl alcohol (70%, v/v) was used as an extractant. Percolation method consisting of three phases: wetting (swelling), infusion and directly percolation was made use of.
Extracts were obtained in two stages. At the first stage we obtained 85% of extract in respect to the weight of raw materials. At the second stage percolation was performed till the complete exhaustion of raw materials. The process of complete exhaustion of raw materials was monitored by yield of esters in terms of valerenic acid ether. The extract obtained at the second stage was evaporated up to 15% in respect to the weight of raw materials. Thus, the hydro-alcohol extract of valerian rhizomes and roots at a ratio of 1:1 was obtained. The obtained extract was purified by settling at a temperature of 8°С for three days and subsequent filtration.
The evaluation of the quality of the obtained extract was conducted according to the following criteria: description, density, relative density, ethanol concentration, dry rest , quantitative determination.
The density of the liquid extract was – 0,892 g/cm3, relative density – 0,894, ethanol concentration – 67,5%, dry rest – 9,18.
It has been proposed to standardize liquid extract of valerian rhizomes and roots by the sum of esters in terms of valerenic acid ether. The content of the sum of esters in terms of valerenic acid ether made up 2,61±0,05%.
Key words: percolation, liquid extract of valerian, valerenic acid, quality assessment.
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