DOI: https://doi.org/10.22263/2312-4156.2016.3.60
Aliakhnovich N.S.*, Yanchanka U.V.**
The effect of provocative test with the allergen on the IgE+CD203c+ blood basophils
*Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University», Vitebsk, Republic of Belarus
**Scientific Research Company Resan, Vitebsk, Republic of Belarus
Vestnik VGMU. 2016;15(3):60-68.
Abstract.
Objectives. To identify and assess the IgE expression on basophils with the help of synthetic peptide p137-142FcFRIR, a fragment of the active site of the high affinity receptor to immunoglobulin E, before and after peroral provocative test with the allergen.
Material and methods. 50 people were enrolled in the study: 29 – with verified allergy – the studied group, 21 – without any allergy - the controls. After obtaining their informed consent the peroral provocative test with the house dust allergen or food dyes: tartrazine and / or titanium dioxide was conducted.
Blood was sampled on an empty stomach before and 40 minutes after the provocative test. Flow cytometry IgE+ and CD203c+ phenotyping was performed using the test system IgE-203c containing synthetic peptide p137-142FcFRIR-FITC and monoclonal antibodies CD203c-PE.
Results. The initial IgE+CD203c+ basophils number is higher in the individuals with verified allergic diseases and allergic reactions to the household and food allergens (p<0,05). The IgE+CD203c+ basophils amount more than 10 per 30,000 blood cells was revealed in 82% cases of the studied group and in 18% controls (p=0,03). After the peroral provocative test in patients with allergies in 78% cases there was the IgE+CD203c+ basophils number change (over 30%): in 30% - increasing, in 48% - decreasing. The IgE+CD203c+ basophils number varied in an inverse proportion to their original amount, a correlation with skin tests was observed. The IgE+CD203c+ basophils level changed more than by 30% after the peroral test with the house dust allergen – in 54% cases; with the food dye – in 83% cases.
Conclusions. The IgE+CD203c+ basophils number in people with allergies is higher than that in healthy individuals and is a significant criterion of allergopathology. Food dyes cause white blood cells phenotype change more often than the house dust allergen. Detection of IgE, associated with the cells, seems to be expedient for IgE-dependent diseases diagnosing in humans.
Key words: provocative test, basophils, immunoglobulin E, IgE+, CD203c+.
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Information about authors:
Aliakhnovich N.S. – senior teacher of the Chair of Clinical Immunology &Allergology with the course of the Faculty for Advanced Training & Retraining, Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University»;
Yanchanka U.V. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Immunology & Allergology with the course of the Faculty for Advanced Training & Retraining, Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University».
Correspondence address: Republic of Belarus, 210023, Vitebsk, 27 Frunze ave., Educational Establishment «Vitebsk State Order of Peoples’ Friendship Medical University», Chair of Clinical Immunology & Allergology with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Aliakhnovich N.S.
