DOI: https://doi.org/10.22263/2312-4156.2016.4.18
Paplauskaya E.A.
Structural features of the interstitial tissue of the rats’ testes on exposure to bacterial lipopolysaccharide S. marcescens
Educational Establishment «Grodno State Medical University», Grodno, Republic of Belarus
Vestnik VGMU. 2016;15(4):18-24.
Abstract.
It has been experimentally proved in this work that a single intraperitoneal injection of lipopolysaccharide Serratia marcescens in a dose of 50 mcg / kg of the body weight in male rats causes the development of a variety of structural changes in the interstitial tissue of the experimental animals testes. While studying histological preparations of the rats’ testes in experimental animals, interstitial edema and an increase in the diameter of peritubular hemocapillaries are registered. In some hemocapillaries hemostasis and leukocytic infiltration are observed. Interstitial endocrinocytes of animals from the experimental group distinguish themselves by polymorphism, are located predominantly in the interstitial groups, and are stellate or oval in their form. The cellular cytoplasm is often microvacuolated with unevenly distributed, and different in tinctorial properties granularity. Furthermore, there is a decrease in the amount of endocrine cells in the testicular interstitial tissue of experimental animals in comparison with that in the control group, cellular nuclei in all investigated periods, according to morphometry data are reduced in size. All of the above-mentioned changes in the interstitial tissue of experimental animals testes persist over a fairly long period of time after exposure to bacterial lipopolysaccharide Serratia marcescens (3rd, 10th, 20th, 30th, 40th and 50th day after administration) and can lead to the disturbance of spermatogenesis processes in animals.
Key words: bacterial lipopolysaccharide, testis, interstitial endocrinocytes.
References
1. Bykov BL. Spermatogenez u muzhchin v kontse XX veka (obzor literatury) [Spermatogenesis at men at the end of the 20th century (the review of literature)]. Problemy Reproduktsii. 2000;(1):6-13.
2. Sofikitis N, Giotitsas N, Tsounapi P, Baltogiannis D, Giannakis D, Pardalidis N. Hormonal regulation of spermatogenesis and spermiogenesis. J Steroid Biochem Mol Biol. 2008 Apr;109(3-5):323-30.
3. de Kretser DM, Loveland KL, Meinhardt A, Simorangkir D, Wreford N. Spermatogenesis. Hum Reprod. 1998 Apr;13 Suppl 1:1-8.
4. Bergh A. Paracrine regulation of Leydig cells by the seminiferous tubules. Int J Androl. 1983 Feb;6(1):57-65.
5. Dudenkova NA, Shubina OS. Izmeneniia morfofunktsional'nogo sostoianiia i produktivnosti semennykh zhelez belykh krys pri vozdeistvii atsetata svintsa [Changes of a morfofunktsionalny state and efficiency of seed glands of white rats at lead acetate influence]. Fundam Issled. 2013;10 ch 6;1253-9.
6. Kidun KA, E. K. Solodova, Ugolnik TS, Doroshenko RV. Stress-indutsirovannye izmeneniia antioksidantnogo statusa spermatozoidov i morfologii semennikov krys [The stress-induced changes of the antioxidatic status of spermatozoons and morphology of spermaries of rats]. Problemy Zdorov'ia i Ekologii. 2014;(2):125-9.
7. Kurlyandskiy BA, Filov VA, red. Obshchaia toksikologiia [General toxicology]. Moscow, RF: Meditsina; 2002. 608 р.
8. Al Meselmani MA. Vliianie gamma-izlucheniia na mitokhondrial'noe okislenie v semennikakh krys [Influence of a gamma radiation on mitochondrial oxidation in spermaries of rats]. Med Zhurn. 2010;(4):17-20.
9. O'Shaughnessy PJ, Morris ID, Huhtaniemi I, Baker PJ, Abel MH. Role of androgen and gonadotrophins in the development and function of the Sertoli cells and Leydig cells: data from mutant and genetically modified mice. Mol Cell Endocrinol. 2009 Jul;306(1-2):2-8.
Information about authors:
Paplauskaya Elena A. – Candidate of Biological Sciences, teacher of the Chair of Histology, Cytology & Embryology, Educational Establishment «Grodno State Medical University».
Correspondence address: Republic of Belarus, 230021, Grodno, 71A Belyje Rosy str., 42. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Paplauskaya Elena A.
