DOI: https://doi.org/10.22263/2312-4156.2016.5.44
Belyaeva L.E., Fedchenko A.N., Lazuko S.S., Ligetskaya I.V., Orekhova N.I.
Gender differences of disorders of coronary vessels’ tone and cardiac contractility in offspring of rats underwent a chronic stress during pregnancy
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2016;15(5):44-51.
Abstract.
Objectives. To estimate the coronary vessels tone and cardiac contractility of isolated by Langendorff method hearts received from 3-month offspring born from rats which underwent chronic stress during their pregnancy.
Material and methods. Experiments were performed on 20 white mongrel Rattus Muridae weighing 200-250 g and their 3-month offspring (n=37). Pregnant rats were exposed to chronic stress combining food deprivation during one day with the provision of free access to water, immobilization in the plastic box filled with water of room temperature, and contact with Felis excrements to imitate a predator presence. Coronary vessels tone and cardiac contractility of their 3-month offspring were studied on the isolated by Langendorff method and isometrically contracted heart which was perfused with standard Krebs-Henseleit solution at the constant coronary flow.
Results. Chronic stress during pregnancy contributed to a decrease of the incremental growth of the coronary perfusion pressure on increasing the coronary flow from 8 to 10 ml/min. and from 10 to 15 ml/min by 14% and 23%, respectively, in the hearts of 3-month male offspring. Left ventricular pressure in isolated hearts from 3-month male rats, whose mothers experienced stress during pregnancy, was on an average by 26% lower than that in the control male offspring. Velocity of the myocardium contraction and relaxation of hearts isolated from male offspring, whose mothers underwent stress during pregnancy, was decreased to the same extent (by 38%) at the coronary flow amounting to 8 ml/min.
Conclusions. Chronic stress exerting influence on the pregnant rats contributes to a decrease of coronary vessels tone and left ventricular pressure, reduction in velocity of the myocardial contraction and relaxation in hearts isolated from male, but not female 3-month offspring. The documented changes may promote the cardiovascular system damage in such male offspring.
Key words: chronic stress, mature offspring, coronary vessels tone, myocardial contractility.
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Information about authors:
Belyaeva L.E. – Candidate of Medical Sciences, associate professor, head of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Fedchenko A.N. – Master of Medical Sciences, teacher of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Lazuko S.S. – Candidate of Biological Sciences, associate professor, head of the Chair of Normal Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Ligetskaya I.V. – teacher of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Orekhova N.I. – MSc (Medicine) student of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210023, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Pathologic Physiology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Belyaeva Lyudmila E.
