DOI: https://doi.org/10.22263/2312-4156.2017.1.16
Asachuk S.S.
The effect of flaxseed oil on the spectrum of fatty acids of sphingomyelins and phosphatidylcholines of erythrocytes membranes in sportsmen
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2017;16(1):16-22.
Abstract.
Sphingomyelins participate in the formation of preprotein pools of the membranes and exert a significant influence on the function of transmembrane proteins. Phosphatidylcholines are major phospholipids of membranes. They are involved in the transport of polyunsaturated fatty acids and cells metabolism. Physico-chemical properties of the membranes are determined by the spectrum of phospholipid fatty acids and determine the functional activity of erythrocytes membranes in athletes.
Objectives. To investigate the effect of flaxseed oil on the spectrum of fatty acids of sphingomyelins and phosphatidylcholines of sportsmen’s erythrocytes membranes.
Material and methods. Linseed-oil obtained from Ltd «Club «Pharm-Eco» (Republic of Belarus, the town of Drogichin), contained 11,2±4,2% palmitic (C16:0), 13,6±5,8% oleic (C18:1n9), 42,2±8,1% linoleic (C18:2n6), 32,2±4,4% linolenic (C18:3n3) acids. The study was conducted on the athletes of the Vitebsk School of Olympic Reserve. The blood from the cubital vein was taken at the beginning of the investigation on the 15th and the 30th day of linseed-oil intake. Sphingomyelins and phosphatidylcholines were isolated from erythrocytes membranes by the thin-layer chromatography (TLC) method. The fatty acid spectrum of phospholipids was determined by gas chromatography with capillary column.
Results. It has been found that the greatest changes occur in sphingomyelins. The amount of oleic (C18:1n9), linoleic (C18:2n6), linolenic (C18:3n3), and arachidonic (C20:4n6) acids was noted to increase. It has been concluded that the synthesis of oleic (C18:1n9) and arachidonic (C20:4n6) acids was increased.
Conclusions. The conclusion about the possibility of a modified sphingomyelins considerable influence on the conformation of the transmembrane proteins and membrane function as a whole has been arrived at.
Key words: polyunsaturated fatty acids, membrane, red blood cell, athletes.
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Information about authors:
Asachuk S.S. – Doctor of Medical Sciences, associate professor, head of the Scientific-Research Laboratory, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210023, Vitebsk, 27/3 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Scientific-Research Laboratory. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Sergey S. Asachuk.
