DOI: https://doi.org/10.22263/2312-4156.2018.1.34
Osipov B.B.1, Kazlou A.Y.2
The effect of ozonotherapy on the indices of oxidative stress and antioxidant mechanisms in experimental liver cirrhosis
1Gomel State Medical University, Gomel, Republic of Belarus
2The Institute of Radiobiology of the Belarusian National Academy of Sciences, Gomel, Republic of Belarus
Vestnik VGMU. 2018;17(1):34-42.
Abstract.
Objectives. To estimate the changes of some biochemical indices of oxidative stress and antioxidant mechanisms in rats with experimental liver cirrhosis and also to evaluate an effect of the course of ozonotherapy on the balance of these indicators in experimental liver cirrhosis.
Material and methods. White male rats of the line Wistar (n=15) served as an object of this research. After modelling liver cirrhosis (by our own technique) a course of ozonotherapy was provided to the rats of the experimental group. Then the animals were taken from the experiment and the markers of oxidative stress and antioxidant mechanisms in the blood serum and liver samples were studied and compared to those of the control animals.
Results. A statistically significant increase of the oxidative stress indices and a decrease in the activity of antioxidant enzymes compared to normal indicators (p=0,009) are observed in rats with experimental liver cirrhosis. The course of ozonotherapy in experimental liver cirrhosis leads to the statistically significant decrease of the majority of the oxidative stress markers (p=0,009) and the increase in the activity of antioxidant enzymes and some non-enzymatic antioxidant mechanisms (p=0,016 for the concentration of protein sulfhydryl groups in the liver tissue and p=0,009 for other indices) in comparison with the control group animals (with liver cirrhosis without any therapeutic influence).
Conclusions. Liver cirrhosis is accompanied by the statistically significant increase of oxidative processes indices and the decreased activity of antioxidant enzymes in comparison with the norm. The course of ozonotherapy has a positive effect on the imbalance between oxidative and antioxidant processes, which is proved by the statistically significant decrease of the majority of the studied oxidative processes markers and the increase of antioxidant enzymes activity.
Key words: liver cirrhosis, ozonotherapy, oxidative stress, antioxidant processes and mechanisms, markers.
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Information about authors:
Osipov B.B. – lecturer of the Chair of Surgical Diseases No.1 with the course of Cardiovascular Surgery, Gomel State Medical University;
Kazlou A.Y. – associate research officer of the Endocrinology & Biochemistry Laboratory, the Institute of Radiobiology of the Belarusian National Academy of Sciences.
Correspondence address: Republic of Belarus, 246050, Gomel, 5 Lange str., Gomel State Medical University, Chair of Surgical Diseases No.1 with the course of Cardiovascular Surgery. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Boris B. Osipov.
