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Osipov B.B.1, Kazlou A.Y.2
The effect of ozonotherapy on the indices of oxidative stress and antioxidant mechanisms in experimental liver cirrhosis
1Gomel State Medical University, Gomel, Republic of Belarus
2The Institute of Radiobiology of the Belarusian National Academy of Sciences, Gomel, Republic of Belarus

Vestnik VGMU. 2018;17(1):34-42.

Objectives. To estimate the changes of some biochemical indices of oxidative stress and antioxidant mechanisms in rats with experimental liver cirrhosis and also to evaluate an effect of the course of ozonotherapy on the balance of these indicators in experimental liver cirrhosis.
Material and methods. White male rats of the line Wistar (n=15) served as an object of this research. After modelling liver cirrhosis (by our own technique) a course of ozonotherapy was provided to the rats of the experimental group. Then the animals were taken from the experiment and the markers of oxidative stress and antioxidant mechanisms in the blood serum and liver samples were studied and compared to those of the control animals.
Results. A statistically significant increase of the oxidative stress indices and a decrease in the activity of antioxidant enzymes compared to normal indicators (p=0,009) are observed in rats with experimental liver cirrhosis. The course of ozonotherapy in experimental liver cirrhosis leads to the statistically significant decrease of the majority of the oxidative stress markers (p=0,009) and the increase in the activity of antioxidant enzymes and some non-enzymatic antioxidant mechanisms (p=0,016 for the concentration of protein sulfhydryl groups in the liver tissue and p=0,009 for other indices) in comparison with the control group animals (with liver cirrhosis without any therapeutic influence).
Conclusions. Liver cirrhosis is accompanied by the statistically significant increase of oxidative processes indices and the decreased  activity of antioxidant enzymes in comparison with the norm. The course of ozonotherapy has a positive effect on the imbalance between oxidative and antioxidant processes, which is proved by the statistically significant decrease of the majority of the studied oxidative processes markers and the increase of antioxidant enzymes activity.
Key words: liver cirrhosis, ozonotherapy, oxidative stress, antioxidant processes and mechanisms, markers.


1. Scaglione S, Kliethermes S, Cao G, Shoham D, Durazo R, Luke A, Volk ML. The Epidemiology of Cirrhosis in the United States: A Population-based Study. J Clin Gastroenterol. 2015 Sep;49(8):690-6. doi:
2. Bobrov AN. Cirrhosis of liver: etiological, epidemiological, clinical, diagnostic and preventive aspects according the 15-year (1996-2010). observations in a multidisciplinary hospital: dis … d-ra med nauk: 14.01.04. Moscow, RF; 2011. 290 р. (In Russ.)
3. Men T, Brennan P, Boffetta P, Zaridze D. Russian mortality trends for 1991-2001: analysis by cause and region. BMJ. 2003 Oct;327(7421):964. doi:
4. Parola M, Robino G. Oxidative stress-related molecules and liver fibrosis. J Hepatol. 2001 Aug;35(2):297-306.
5. Rolo AP, Teodoro JS, Palmeira CM. Role of oxidative stress in the pathogenesis of nonalcoholic steatohepatitis. Free Radic Biol Med. 2012 Jan;52(1):59-69. doi:
6. Bulatova IA, Shchekotova AP, Suzdal'tseva KN, Shchekotov VV, Ulitina PV, Zhizhilev EV. Superoxide dismutase and glutathionereductase in chronic hepatitis C and nonalcoholic fatty liver disease. Fundam Issled. 2014;(7-3):455-9. (In Russ.)
7. Kontorshchikova KN. Regulatory effects of ozone. Nizhegorod Med Zhurn. 2003;(S):5-6. (In Russ.)
8. Rilling S. 30 years of ozone-oxygen therapy a historical perspective. In: Ozone in medicine: proceedings of the eleventh Ozone World Congress Aug 29 – Sep 3 1993 San Francisco. San-Francisco, USA; 1993. P. 1-14.
9. Taganovich AD, Oletskiy EI, Kotovich IL. Pathological biochemistry. Moscow, RF: BINOM; 2013. 447 р. (In Russ.)
10. Malakhov VA, Belous AM, Pasyura IN, Doroshenko GI. Cellular and metabolic aspects of the pathogenesis, treatment and prevention of chronic cerebral ischemia and neurodegenerative processes. Kharkov, Ukraine: Osnova; 2000. 174 р. (In Russ.)
11. Bocci V. Ozone as a bioregulator. Pharmacology and toxicology of ozonetherapy today. J Biol Regul Homeost Agents. 1996 Apr-Sep;10(2-3):31-53.
12. Kontorschikova CN. Biochemical safety control in ozone therapy. In: Ozone in Medicine: 12th World Congress of the International Ozone Association. Lille: Tours-Instaprint; 1995. Vol 3. P. 231-4.
13. Viebahn-Haensler R, Fernandez O, Fahmy Z. Ozone in Medicine: Clinical Evaluation and Evidence Classification of the Systemic Ozone Applications, Major Autohemotherapy and Rectal Insufflation, According to the Requirements for Evidence-Based Medicine. Ozone: Science & Engineering. 2016;38(5):322-45.
14. Riva Sanseverino E, Castellacci E, Castellacci P. Oxygen-ozone therapy and physical activity in humans. In: Proceedings of the 12-th World Congress of the International Ozone Association. Zurich; 1995. Vol 3. Р. 65-72.
15. Fazylov VKh, Galeeva NV, Zagidullina AI, Tairov IN. Ozone therapy at the clinic of infectious diseases. Prakt Meditsina. 2013;(5):47-51. (In Russ.)
16. Hotchkiss JA, Harkema JR. Endotoxin or cytokines attenuate ozone-induced DNA synthesis in rat nasal transitional epithelium. Toxicol Appl Pharmacol. 1992 Jun;114(2):182-7.
17. Gazin IK. Evaluation of some hemostatic parameters in patients with complicated diabetes if you are using ozone therapy. Efferent Terapiia. 2001;7(2):67-8. (In Russ.)
18. Shlafer MB, Shepard M. A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances. Anal Biochem. 1984 Mar;137(2):269-76.
19. Taylor EL, Armstrong KR, Perrett D, Hattersley AT, Winyard PG. Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus. Oxid Med Cell Longev. 2015;2015:496271.
20. Kapaki E, Liappas I, Lyras L, Paraskevas GP, Mamali I, Theotoka I, et al. Oxidative damage to plasma proteins in patients with chronic alcohol dependence: the effect of smoking. In Vivo. 2007 May-Jun;21(3):523-8.
21. Moin VM. A simple and specific method for the determination of glutathione peroxidase activity in erythrocytes. Lab Delo. 1986;(12):724-7. (In Russ.)
22. Grintzalis K, Zisimopoulos D, Grune T, Weber D, Georgiou CD. Method for the simultaneous determination of free/protein malondialdehyde and lipid/protein hydroperoxides. Free Radic Biol Med. 2013 Jun;59:27-35. doi:
23. Mannervik B, Jemth P. Measurement of Glutathione Transferases. Curr Protoc Toxicol. 2001;Chapter 6:Unit6.4. doi:
24. Sirota TV. The use of nitro blue tetrazolium in the reaction of autookislenia of adrenaline to determine the activity of superoxide dismutase. Biomed Khimiia. 2013;59(4):399-410. (In Russ.)
25. Green RM, Graham M, O'Donovan MR, Chipman JK, Hodges NJ. Subcellular compartmentalization of glutathione: Correlations with parameters of oxidative stress related to genotoxicity. Mutagenesis. 2006 Nov;21(6):383-90. doi:
26. Filchenkov GN, Popoff EH, Naumov AD. The low dose gamma ionising radiation impact upon cooperativity of androgenspecific proteins. J Environ Radioact. 2014 Jan;127:182-90. doi:
27. Bissvanger Kh, Mosolova T, per. Practical Enzymology. Moscow, RF: BINOM Laboratoriia znanii; 2015. 328 р. (In Russ.)

Information about authors:
Osipov B.B. – lecturer of the Chair of Surgical Diseases No.1 with the course of Cardiovascular Surgery, Gomel State Medical University;
Kazlou A.Y. – associate research officer of the Endocrinology & Biochemistry Laboratory, the Institute of Radiobiology of the Belarusian National Academy of Sciences.

Correspondence address: Republic of Belarus, 246050, Gomel, 5 Lange str., Gomel State Medical University, Chair of Surgical Diseases No.1 with the course of Cardiovascular Surgery. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Boris B. Osipov.

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