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Horehliad O.M.1,2
The change of wound contamination under the influence of local application of negative pressure
1Dnepropetrovsk Medical Academy of the Ukrainian Ministry of Public Health, Dnepr, Ukraine
2Dnepr Clinical Hospital Named after I.I. Mechnicov, Dnepr, Ukraine

Vestnik VGMU. 2018;17(2):63-69.

Objectives. To investigate the effect of vacuum-assisted closure (VAC) of the wounds on their bacterial load in patients with severe polystructural high-energy injuries.
Material and methods. 34 cases of severe osteomuscular injuries of the lower and upper extremities were examined. 18 patients were included into the experimental group with the application of negative pressure on the wound. Standard wound treatment with antiseptics and the application of gauze bandages were used in 16 patients of the control group. The evaluation of the results included the comparison of the bacterial load (the amount of colony forming units per gram of tissue) during the wound condition examination in the experimental and control groups. The specimens for bacteriological evaluation, which included the determination of the qualitative and quantitative composition of wound microbiocenosis, were collected from the wound bed before the application of treatment measures as well as 3 and 7 days later.
Results. The total bacterial load after the negative pressure wound treatment was significantly lower than that after the conventional wound treatment at each observation time period, whereas the bacterial load in the wound treated by means of the conventional saline-soaked gauze bandage was significantly lower after 7 days had elapsed than that in 3 days after application. The load of anaerobic bacteria was equally perceptible for both VAC and control group treatment, whereas the load of P. aeruginosa and S. aureus was significantly decreased even in 3 days after negative pressure application.
Conclusions. The application of wounds VAC leads to a significant decrease in the bacterial load of the wound, which means that negative pressure wound healing significantly reduces the level of wounds contamination, thus it can be applied for the successful treatment of complex polystructural wounds.
Key words: wound healing, vacuum-assisted closure, combat wound, polystructural injury, bacterial load, wound infection.


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Information about authors:
Horehliad O.M. – orthopedist-traumatologist, head of the Consultation Polyclinic, Dnepr Clinical Hospital Named after I.I. Mechnikov; postgraduate of the Chair of Medicosocial Expertise & Rehabilitation, Dnepropetrovsk Medical Academy of the Ukrainian Ministry of Public Health.

Correspondence address: Ukraine, 49005, Dnepr, 14 Sobornaya sq., Dnepr Clinical Hospital Named after I.I. Mechnicov. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Oleksii M. Horehliad Oleksii M. Horehliad.

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