Masyuk N.Y., Gorodetskaya I.V.
The correlation analysis of the concentration of iodine-containing thyroid hormones in the blood, caries intensity and the activity of lipid peroxidation in the saliva of rats
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2018;17(3):38-43.
Objectives. To identify and assess the degree of correlation between the concentration of iodine-containing thyroid hormones in the blood and the activity of the carious process in the enamel and dentin, lipid peroxidation intensity and antioxidant activity in the saliva of rats under the conditions of cariogenic effects.
Material and methods. Mercazolil was administered at the dose of 25 mg/kg during 30 days, then up to 60 days the half dose was given, L-thyroxine – in low doses (1,5-3,0 mg/kg during 28 days, then up to 60 days 1,5 mg/kg was given). As cariogenic factors we used the Stephen diet (during 60 days), crowding stress (40 rats kept in one cage during 1 month, 30 rats during the second month) and their combination. The expressiveness of the carious process was evaluated by the prevalence, frequency, severity and depth of the lesion. The level of iodine-containing thyroid hormones was investigated by means of enzyme immunoassay using special diagnostic kits. The activity of lipid peroxidation and antioxidant systems in the saliva was determined by chemiluminescent method. The correlation analysis of the obtained data was carried out using Statistica 10.0 program.
Results. The resistance of the enamel and dentin to carious lesions depends on the serum concentration of iodine-containing thyroid hormones due to their influence on the intensity of lipid peroxidation in the saliva and its antioxidant activity.
Key words: iodine-containing thyroid hormones, stress, tooth decay, lipid peroxidation, correlation.
1. Muchandi S, Walimbe H, Bijle MN, Nankar M, Chaturvedi S, Karekar P. Comparative evaluation and correlation of salivary total antioxidant capacity and salivary pH in caries-free and severe early childhood caries children. J Contemp Dent Pract. 2015 Mar;16(3):234-7.
2. Ziaudeen S, Ravindran R. Assessment of oxidant-antioxidant status and stress factor in recurrent aphthous stomatitis patients: case control study. J Clin Diagn Res. 2017 Mar;11(3):ZC01-ZC04. doi: http://dx.doi.org/10.7860/JCDR/2017/22894.9348
3. Mejía-Rubalcava C, Alanís-Tavira J, Argueta-Figueroa L, Legorreta-Reyna A. Academic stress as a risk factor for dental caries. Int Dent J. 2012 Jun;62(3):127-31. doi: http://dx.doi.org/10.1111/j.1875-595X.2011.00103.x
4. Mayboroda YuN, Goman MV, Khorev OYu. Effect of the drug "Mexidol" on the state of lipid peroxidation and antioxidant activity of saliva in patients with periodontitis on the background of hypothyroidism. Kuban Nauch Med Vestn. 2014;(4):74-8. (In Russ.)
5. Stephan RM. Effects of different types of human foods on dental health in experimental animals. J Dent Res. 1966 Sep-Oct;45(5):1551-61. doi: http://dx.doi.org/10.1177/00220345660450054701
6. Donskiy GI, Pavlyuchenko ON, Melikov AV; Donets Gos Med Un-t Im M Gor'kogo. Pat SU 1720082 SSSR, G 09 B 23/28. A method of modeling dental caries. № 4781223/14; zaiavl 10.01.90; opubl 15.03.92, Biul. № 10. 3 р. (In Russ.)
7. Kuz'mina EI, Nelyubin AS, Shchennikova MK. The use of induced chemiluminescence to evaluate free radical reactions in biological substrates. V: Biokhimii i biofiziki mikroorganizmov: mezhvuz sb. Gor'kii, RF; 1983. Р. 179-83. (In Russ.)
8. Rebrova OYu. Statistical analysis of medical data. The use of applied programs Statistica. Moscow, RF: MediaSfera; 2002. 312 р. (In Russ.)
Information about authors:
Masyuk N.Y. – postgraduate of the Chair of Normal Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Gorodetskaya I.V. – Doctor of Medical Sciences, professor of the Chair of Normal Physiology, dean of the Medical Faculty, Vitebsk State Order of Peoples’ Friendship Medical University.