DOI: https://doi.org/10.22263/2312-4156.2018.3.58
Goncharova A.I.
Biofilm forming microorganisms and oral fluid enzymes in the pathogenesis of sialadenitis
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2018;17(3):58-66.
Abstract.
Objectives. To study the etiological structure, the level of sIgA in the oral fluid and IL-1β in the blood serum, the enzymatic activity of the oral fluid in patients with sialadenitis.
Material and methods. 86 patients with sialadenitis were examined. These patients underwent inpatient treatment in the Vitebsk Regional Clinical Hospital. The isolation and identification of ductal saliva microorganisms in patients with sialadenitis were carried out, the ability of isolates to form biofilms was determined, sensitivity assessment of the isolated microorganisms to antibiotics in biofilm was performed; the ability of patients’ blood serum to destroy the biofilm matrix was also studied; the elastase and BAPNA amidase activity of the oral fluid was determined; the IL-1β content in the blood serum was evaluated and the level of sIgA in the oral fluid of patients with sialadenitis was determined.
Results. In 77,9 per cent of cases, the value of the microbial factor was confirmed, among the isolated isolates, representatives of the genus Streptococcus (45,8%) and representatives of the genus Staphylococcus (32,9%) prevailed. The greatest ability to form a microbial film among the frequently occurring isolates belonged to representatives of the species S. aureus – 24,45; 20,27-49.29 μg / socket. While comparing the MIK90 S. aureus in plankton forms and biofilms it was found increased from 8,5 to 99 times. The most effective against S. aureus are imipenem, meropenem, vancomycin and ciprofloxacin. A predisposing risk factor for the development of sialadenitis is a decrease in sIgA in the oral fluid ≤ 0,16 mg / ml, along with a new risk factor for the development of inflammatory diseases of the large salivary glands, such as the low ability to cleave the biofilm matrix – ≤ 11,28 mkg / ml. However, the most significant out of the studied factors is the use of determining the activity level of the oral fluid elastase ≥ 0,005 pc as an additional diagnostic criterion for the development of sialadenitis.
Conclusions. The etiological structure, the level of sIgA in the oral fluid and IL-1β in the blood serum, the enzymatic activity of the oral fluid of patients with sialadenitis were studied.
Key words: sialadenitis, biofilm, elastase activity, BAPNA amidase activity, IL-1β, sIgA.
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Information about authors:
Goncharova A.I. – postgraduate of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210023, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Clinical Microbiology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anna I. Goncharova.
