DOI: https://doi.org/10.22263/2312-4156.2018.4.37
Tkachenko A.S.1, Zhukov V.I.1, Gubina-Vakulyck G.I.1, Nakonechna O.A.1, Gorbach T.V.1, Onishchenko A.I.1, Tkachenko M.A.2
Experimental study of carrageenan-induced gastroenterocolitis pathogenesis
1Kharkov National Medical University, Kharkov, Ukraine
2Kharkov Municipal Clinical Hospital No. 27, Kharkov, Ukraine
Vestnik VGMU. 2018;17(4):37-47.
Abstract.
Objectives. To analyze possible mechanisms for the development of experimental carrageenan-induced gastroenterocolitis in laboratory animals.
Material and methods. Adult female rats were administered carrageenan during 2 and 4 weeks orally. Blood serum levels of TBA-reactive substances and diene conjugates, carbonylated proteins, total antioxidant status, catalase and superoxide dismutase activity, the content of cytokines, caspase-3, matrix metalloproteinase-2, vascular endothelial growth factor (VEGF) and sFASL were determined. The activity of poly (ADP-ribose) polymerase, apoptosis signal-regulating protein kinase-1 (ASK-1), inducible and endothelial NO-synthases, levels of DNA fragmentation, endothelin-1 were measured in the small intestine homogenate. The blood serum protein spectrum was assessed. Morphological and immunohistochemical studies of the small intestine were carried out using antibodies to the Ki-67 antigen.
Results. It has been found that the consumption of carrageenan results in the development of gastroenterocolitis. The disease is accompanied by the development of oxidative stress, activation of enterocyte apoptosis with compensatory inadequate activation of enterocyte proliferation, abnormal serum cytokine spectrum, increased permeability of the small intestinal epithelial barrier, endothelial dysfunction of the small intestinal vessels, and neoangiogenesis.
Conclusions. The impairment of redox homeostasis occurring under the action of carrageenan and overproduction of proinflammatory cytokines by leukocytes are believed to be triggers for the development of carrageenan-induced gastroenterocolitis.
Key words: food additives, carrageenan, carrageenan-induced inflammation, pathogenesis, gastroenterocolitis.
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Information about authors:
Tkachenko A.S. – Candidate of Medical Sciences, lecturer of the Chair of Biochemistry, Kharkov National Medical University;
Zhukov V.I. – Doctor of Medical Sciences, professor of the Chair of Biochemistry, Kharkov National Medical University;
Gubina-Vakulyck G.I. – Doctor of Medical Sciences, professor of the Chair of Pathological Anatomy, Kharkov National Medical University;
Nakonechna O.A. – Doctor of Medical Sciences, professor, head of the Chair of Biochemistry, Kharkov National Medical University;
Gorbach T.V. – Candidate of Biological Sciences, associate professor of the Chair of Biochemistry, Kharkov National Medical University;
Onishchenko A.I. – postgraduate of the Chair of Biochemistry, Kharkov National Medical University;
Tkachenko M.A. – intern, Kharkov Municipal Clinical Hospital No. 27.
Correspondence address: Ukraine, 61022, Kharkov, 4 Nauky ave., Kharkov National Medical University, Chair of Biochemistry. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anton S. Tkachenko.
