DOI: https://doi.org/10.22263/2312-4156.2018.5.102
Basalai O.N., Bushma M.I., Borisenok О.A., Aliferovich U.S.
Correlation interrelationships between nephrotoxicity indices in rats with sublimate nephropathy, receiving taucin
Grodno State Medical University, Grodno, Republic of Belarus
Vestnik VGMU. 2018;17(5):102-108.
Abstract.
Objectives. To carry out the correlation analysis of interrelations between morphological, histochemical and biochemical indicators of nephrotoxicity in rats with sublimate nephropathy without treatment and receiving taucin.
Experiments were made on 24 not purebred male rats weighing 200-250 g which were divided into 3 groups (n=8). The first group was the control one; the second – experiment 1 (animals receiving sublimate, intraperitoneally in the dose of 0.1 mg/kg/day); the third – experiment 2 (animals receiving sublimate, intraperitoneally in the dose of 0.1 mg/kg/day and a combination of taurine with zinc diaspartate (taucin) intragastrically – in the dose of 500 mg/kg/day).
The analysis of correlation interrelations in rats with sublimate nephropathy demonstrates that sharp increase in plasma and synchronous decrease in the urine of marker indicators of nephropathy (content of urea and creatinine, clearance of the latter) substantially are associated with the damage of mitochondria of epithelial cells of proximal convoluted tubules of cortical nephrons (PCT CN), the extent of “wrinkling” of the kidney body and the severity of intratubular hydronephrosis. Under the influence of taucin the nature of interrelations changes not unambiguously. Their greater part disappears and the new ones that were absent appear. The set of these changes demonstrates its nephroprotective action caused to a greater extent by the dominating component – taurine and strengthened with zinc diaspartate. These results supplement and deepen the data on its nephroprotective action which were earlier obtained by us. On their basis, according to the results of routine laboratory investigations of plasma and urine, it is possible to predict the character and severity degree of morphological manifestations of sublimate nephropathy and nephroprotective action of taucin.
Key words: rats, sublimate nephropathy, a combination of taurine with zinc diaspartate, nephroproteсtive action.
References
1. Basalay ON, Mikhal'chuk ECh, Bushma MI, Zimatkin SM. Correction of a combination of taurine with zinc daspartate disorders of kidney structure in rats with sublimate nephropathy. Toksikol Vestn. 2015;(2):31-4. (In Russ.)
2. Basalay ON, Senchuk VV, Kravchuk RI, Bushma MI, Mikhal'chuk ECh, Zimatkin SM, i dr. Cytoprotective effect of combination of taurine with zinc daspartate in rats with lesions of the tubules of the nephron sublimate. Med Novosti. 2015;(6): 73-5. (In Russ.)
3. Mozheyko LA. Classical methods of coloring in histology. V: Zimatkin SM, red. Metody issledovaniia v gistologii. Grodno: GrGMU; 2010. Р. 23-34. (In Russ.)
4. Millonig GA. Advantages of a phosphate buffer for osmium tetroxide solutions in fixation. J Appl Рhysics. 1961;32(1):1637-43.
5. Pirs E, Portugalov VV, red. Histochemistry theoretical and applied. Moscow, RF: Izd-vo inostr lit; 1962. 966 р. (In Russ.)
6. Kamyshnikov VS. Handbook of clinical and biochemical studies and laboratory diagnostics. 3-e izd. Moscow, RF: MEDpress-inform; 2009. 896 р. (In Russ.)
7. Giusto GD, Torres AM. Organic anion transporter 5 renal expression and urinary excretion in rats exposed to mercuric chloride: a potential biomarker of mercury-induced nephropathy. Arch Toxicol. 2010 Oct;84(10):741-9. doi: http://dx.doi.org/10.1007/s00204-010-0541-9
8. Adamyan TsI, Gevorkyan ES, Voskanyan AV, Minasyan SM. Antitoxic effect of taurine. Patol Fiziologiia Eksperim Terapiia. 2012;(2):39-41. (In Russ.)
9. Ogay MA, Stepanova EF, Kholodov DB, Nikolaevskiy VA. Antioxidant and membrane stabilizing effect of taurine. Vestn VGU Ser Khimiia Biologiia Farmatsiia. 2011;(1):186-91. (In Russ.)
10. Bidri M, Choay P. Taurine: a particular aminoacid with multiple functions. Ann Pharm Fr. 2003 Nov;61(6):385-91.
11. Kutyakov VA, Shestakova LA, Salmina AB, Chikun VI. Features of metallothionein expression in rat organs during zinc and lead intoxication. Sib Med Obozrenie. 2014;(2):29-33. (In Russ.)
12. Sheybak VM, Sheybak LN. Biological role of zinc and prospects of medical use of zinc-containing preparations. Grodno, RB: GrGMU; 2003. 84 р. (In Russ.)
13. Williams RJ. Zinc in evolution. J Inorg Biochem. 2012 Jun;111:104-9.
Information about authors:
Basalai O. N. – Candidate of Medical Sciences, senior lecturer of the Chair of Pharmacology Named after Professor M.V. Korablev, Grodno State Medical University;
Bushma M. I. – Doctor of Medical Sciences, professor of the Chair of Pharmacology Named after Professor M.V. Korablev, Grodno State Medical University;
Borisenok O. A. – Candidate of Medical Sciences, associate professor of the Chair of Pharmacology Named after Professor M.V. Korablev, Grodno State Medical University;
Aliferovich U. S. – the fourth-year pediatric faculty student, Grodno State Medical University.
Correspondence address: Republic of Belarus, 230005, Grodno, 4 Bolshaya Troitskaya str., Grodno State Medical University, Chair of Pharmacology Named after Professor M.V. Korablev. Е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. –Olga N.Basalai.
