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Lukashou R.I.
The influence of nature and concentration of organic extractants on flavonoids extraction from calendula flowers
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus

Vestnik VGMU. 2018;17(5):109-123.

Objectives. To study the influence of nature and concentration (volume proportions) of organic extractants on the extraction of flavonoids from calendula flowers.
Material and methods. The object of the study was calendula flowers of the industrial series. The method used in the investigation was high-performance liquid chromatography. The list of organic solvents used in the work is given below. The following volume proportions of these solvents were studied: 20, 40, 60, 80 and 100%.
Results. The results of the study of flavonoids extraction from calendula flowers with the use of methanol, ethanol, propanol-1, acetone, dimethylsulfoxide and their aqueous solutions, as well as water, propanol-2, butanol-1, butanol-2, ethyl acetate are presented in this article. From one to eight flavonoids were detected in the prepared extracts by the method of high-performance liquid chromatography, isoramnetin derivatives predominated. The extracting ability of absolute extractants with regard to flavonoids of calendula flowers decreased in the series: water> methanol> dimethylsulfoxide> ethanol> butanol-1> ethyl acetate> butanol-2> acetone> propanol-1> propanol-2. The extracting ability of propanol-1 and acetone was lower compared with that of their aqueous solutions. The extracting ability of methanol, ethanol and dimethylsulfoxide was comparable with that of their aqueous solutions. Dielectric permeability, dynamic viscosity, boiling point, volume proportions of extractants in solutions statistically significantly (p<0,05) influenced the area of chromatographic peaks of flavonoids. The most critical factor was the volume proportion of ethanol in the solution. It is recommended to use 60% acetone for further quantitative determination of flavonoids in calendula flowers taking into account the significant extractive capacity and good reproducibility of the quantitative determination results.
Conclusions. The variability of qualitative and quantitative composition of extracts from calendula flowers was studied during extraction by means of various extractants.
Key words: calendula flowers, flavonoids, organic extractants and their volume proportions, 60% acetone.


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Information about authors:
Lukashou R.I. – Candidate of Pharmaceutical Sciences, associate professor of the Chair of Standardization of Medicinal Agents with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.
ORCID 0000-0001-5234-6319.

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Standardization of Medicinal Agents with the course of the Faculty for Advanced Training & Retraining E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Raman I. Lukashou.

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