DOI: https://doi.org/10.22263/2312-4156.2018.6.77
Klymenko V.A., Drobova N.N.
Cystic fibrosis course in children depending on the mutations of interleukin-4 and interleukin-10 inflammation modificator genes
Kharkov National Medical University, Kharkov, Ukraine
Vestnik VGMU. 2018;17(6):77-84.
Abstract.
Objectives. To improve the provision of medical care for patients with cystic fibrosis (CF) by clarifying the pathogenetic role of the interleukin-4 (IL) and IL-10 inflammation modificator genes polymorphism in the course of the disease.
Material and methods. Forty-two children with CF were examined. The examination was carried out by means of the standard methods in the remission period. The determination of C589T polymorphism of the IL-4 gene and G1082A polymorphism of the IL-10 gene was carried out using polymerase chain reaction in real time. DNA was isolated from buccal epithelium cells.
Results. Clinical and paraclinical features were studied in the main group of children with IL-4 gene and IL-10 gene mutations (homozygous or heterozygous) and compared to those of patients without any mutations in these genes (control group). The early manifestation of gastrointestinal symptoms (in an infant period predominantly), more severe course of CF (with early Staphylococcus aureus (83,4% vs 35,2%; p<0,05) and Candida albicans (66,7% vs 29,4%; p<0,05) colonization of the tracheobronchial tree, bronchiectasis (75,0% vs 5,8%; p<0,05) and lung fibrosis (91,6% vs 41,2%; p<0,05), severe liver lesions (50% vs 0%; p<0,05)) were found. The immune status was characterized by the increase of the total serum IgE 389,55 (46,42; 662,53) IU/ml, the decrease in the level of spontaneous nitroblue tetrazolium test and spontaneous index of activated neutrophils test.
Conclusions. CF phenotype associated with C589T polymorphism of the IL-4 gene and G1082A polymorphism of the IL-10 gene has been characterized.
Key words: children, cystic fibrosis, clinical and paraclinical examination, polymorphism, interleukin-4 gene, interleukin-10 gene.
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Information about authors:
Klymenko V.A. – Doctor of Medical Sciences, professor, head of the Chair of Pediatrics Propedeutics No. 2, Kharkov National Medical University;
ORCID: https://orcid.org/0000-0002-6762-9650
Drobova N.N. – postgraduate of the Chair of Pediatrics Propedeutics No. 2, Kharkov National Medical University.
ORCID: https://orcid.org/0000-0002-7493-5701
Correspondence address: Ukraine, 61022, Kharkov, 4 Nauky ave., Kharkov National Medical University, Chair of Pediatrics Propedeutics No. 2. е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Nadezhda N. Drobova.
