Emelyanchik S.V.1, Karnyushko O.A.2, Zimatkin S.M.2
Immunohistochemistry of calbindine in the neurons of the cerebral and cerebellar cortex of rats in cholestasis
1Grodno State University named after Yanka Kupala, Grodno, Republic of Belarus
2Grodno State Medical University, Grodno, Republic of Belarus
Vestnik VGMU. 2019;18(1):21-29.
Objectives. To estimate immunoreactivity of the calbindine-D28k protein in the neurons of the cerebral and cerebellar cortex of rats after the ligation of the common bile duct (CBD), as a model of subhepatic cholestasis.
Material and methods. The immunohistochemical investigation was carried out on 36 white male rats weighing 200-250 g. Immunoreactivity of the calbindine-D28k protein was determined on paraffin sections in the neurons of the parietal, frontal cortex and cerebellum cortex in 2, 5, 10, 20, 45 and 90 days after the CBD ligation.
Results. It has been established that in the conditions of cholestasis increase caused by the CBD ligation, immunoreactivity of calbindine-D28k in the neurons of the rats’ cerebral and cerebellar cortex grew with its maximum on the 10th-20th day of the experiment. When cholestasis is eliminated as a result of bypass bile ducts growth, immunoreactivity of calbindine in the cerebral cortex neurons becomes normal on the 45th-90th day after the CBD ligation, but it remains moderately elevated in Purkinje cells of the cerebellum. The most pronounced changes of the calbindine immunoreactivity in cholestasis have been noted in the cerebellum Purkinje cells and internal pyramidal neurons of the cerebral cortex.
Conclusions. In cholestasis caused by the CBD ligation, immunoreactivity of calbindine-D28k in the neurons of the rats’ cerebral and cerebellar cortex gradually increases and on cholestasis elimination it is partially normalized. This testifies to the important role of this calcium-binding protein in the processes of cortical neurons adaptation in subhepatic cholestasis.
Key words: calbindine-D28k, neurons, cerebral cortex, cerebellum cortex, cholestasis, rats.
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Information about authors:
Emelyanchik S.V. – Candidate of Medical Sciences, associate professor of the Chair of Zoology & Human and Animal Physiology, Grodno State University named after Yanka Kupala;
Karnyushko O.A. – Candidate of Biological Sciences, lecturer of the Chair of Histology, Cytology & Embryology, Grodno State Medical University;
Zimatkin S. M. – Doctor of Biological Sciences, professor, head of the Chair of Histology, Cytology & Embryology, Grodno State Medical University.