Zaychenko G.V., Pokotylo O.A.
Experimental study of pharmacokinetic profile of the cream with cerium dioxide nanoparticles
Bogomolets National Medical University, Kiev, Ukraine
Vestnik VGMU. 2019;18(1):59-64.
Objectives. To study some parameters of pharmacokinetics of the cream with cerium dioxide nanoparticles (CDN) on single skin application in rats.
Material and methods. CDN were synthesized in LLC «NanoMedTech», the dermal cream with 0,25% CDN was developed in NTC «Institute for Monocrystals» of NAS of Ukraine. The cream with CDN was applied in rats epicutaneously in the dose of 2 mg/cm2 according to the groups: 1) intact control; 2–6) application of the cream with subsequent euthanasia after 2, 6, 12, 24, and 48 hours; 7–11) application of the cream with sudsequent ultraviolet (UV) exposure and euthanasia after 2, 6, 12, 24, and 48 hours. Blood and application site skin samples, as well as the liver, kidneys, and the spleen, were taken after euthanasia. The cerium concentration was measured by means of inductively coupled plasma atomic emission spectroscopy method.
Results. No cerium was detected in the blood and internal organs of rats, even after UV exposure. An accumulation of CDN in the skin without concentration changes was observed during 24 hours. There were no changes of the studied parameter value in 24 hours on comparing groups with or without UV exposure, which indicates an absence of UV effect on absorption of CDN.
Conclusions. On single skin application of the cream with CDN in the dose of 2 mg/cm2 in rats, cerium is accumulated in the skin during 24 hours with further decrease in concentration, it isn’t found in the blood and internal organs, which suggests the presence of a long-term photoprotective effect of CDN without resorptive action.
Key words: cerium dioxide nanoparticles, pharmacokinetics, skin application, photodynamic injury, rats.
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Information about authors:
Zaychenko G.V. – Doctor of Medical Sciences, professor, head of the Chair of Pharmacology, Bogomolets National Medical University;
Pokotylo O.A. – postgraduate of the Chair of Pharmacology, Bogomolets National Medical University.