DOI: https://doi.org/10.22263/2312-4156.2019.2.37
Sheibak V.M., Pauliukavets A.Y., Doroshenko E.M., Olekhnovich E.A.
Acute effect of single introduction of taurine: specific or nonspecific?
Grodno State Medical University, Grodno, Republic of Belarus
Vestnik VGMU. 2019;18(2):37-43.
Abstract.
Objectives. To reveal the dynamic changes in the amino acid pool of the plasma after a single injection of a pharmacological dose of taurine.
Material and methods. The experiment was performed on 29 female rats weighing 120-140 g, with free access of animals to food and water. Taurine was administered to rats intragastrically at a dose of 500 mg / kg of body weight. Animals were decapitated in 15, 30, and 90 min after taurine administration. The blood plasma was used for analysis. Determination of free amino acids was carried out by the method of reversed-phase HPLC.
Results. A single intragastric administration of taurine (500 mg/kg) leads to an increase in the concentration of this amino acid in the blood plasma, the maximum level of which was recorded after 30 min (837.5±45.89 μmol / l, whereas in the control it was 142.0±18.95 μmol/l), however, the most pronounced changes in the amino acid pool of the blood plasma were observed in 90 minutes after taurine administration. A single administration of taurine reduced the total amount of amino acids and their nitrogen-containing metabolites in the blood plasma.
Conclusions. Thus, a decrease in the total amount of amino acids and their nitrogen-containing derivatives in rats’ blood plasma, caused by the administration of taurine, is likely to indicate a nonspecific stimulation of protein synthesis.
Obviously, the osmoregulatory, antioxidant and hormonal effects of taurine will be affected to the greatest extent by the concentration-dependent changes of amino acids in the blood plasma and extracellular fluid, while long-term administration of it in small (close to physiological) doses largely implies a more subtle effect on the signal / regulatory mechanisms.
Key words: taurine, free amino acids, blood plasma, rats, nitrogen-containing metabolites of amino acids.
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Information about authors:
Sheibak V.M. – Doctor of Medical Sciences, professor of the Chair of Biologic Chemistry, Grodno State Medical University;
Pauliukavets A.Y. – Candidate of Biological Sciences, associate professor of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg, Grodno State Medical University;
Doroshenko E.M. – Candidate of Biological Sciences, associate professor, leading research officer of the Scientific-Research Laboratory, Grodno State Medical University;
Olekhnovich E.A. – the fifth-year medical student, Grodno State Medical University.
Correspondence address: Republic of Belarus, 230023, Grodno, 19 Vilenskaya str., Grodno State Medical University, Chair of Microbiology, Virology and Immunology named after S.I. Gelberg. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anastasiya Y. Pauliukavets.
