DOI: https://doi.org/10.22263/2312-4156.2019.3.75
Adaskevich U.P., Miadzelets M.O., Stakhovich I.I., Generalov I.I.
The possible role of fusiform bacteria in the etiopathogenesis of perioral dermatitis
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2019;18(3):75-80.
Abstract.
The role of microbial factors in the development of perioral dermatitis (PD) has been actively studied recently. Previously PD was believed to be connected with candidiasis but lately reports have increasingly appeared about its tentative association with infections caused by fusiform bacteria. The researchers in this case are guided, as a rule, by standard microbiological techniques such as light microscopy and cultivation of biologic materials on various nutrient media.
This study was aimed at clarifying the role of fusiform bacteria in the development of PD with the help of both microscopic and other advanced diagnostic methods, PCR in particular. We examined 30 patients with facial dermatoses (10 with PD, 10 with papulopustular subtype of rosacea and 10 with seborrheic dermatitis), as well as 10 healthy subjects constituting the control group.
Microscopic investigation resulted in detecting fusiform rod-shaped bacteria containg some dark coloured granules within the pale cytoplasm in the majority of patients with PD (80% of cases), as well as in 40% of patients with rosacea, 40% of patients with seborrheic dermatitis, and in 10% of healthy subjects from the control group. The investigation by means of the real-time PCR techniques detected fusobacteria (Fusobacterium nucleatum ssp) in the equal quantity of patients in all groups (50%), and also in one healthy subject from the control group.
The results of our investigations conducted with the help of microscopy and the real-time PCR techniques did not confirm the interrelationship between the detecting of fusobacteria and the pathogenesis of PD.
Key words: perioral dermatitis, fusiform bacteria, role in the etiopathogenesis.
References
1. Dirschka T, Weber K. Topical cosmetics and perioral dermatitis. J Dtsch Dermatol Ges. 2004 Mar;2(3):194-9.
2. Grashkin VA. Role of endogenous intoxication in the pathogenesis of perioral dermatitis. Vestn Poslediplom Obrazovaniia. 2004;(2):25-6. (In Russ.)
3. Dovzhanskiy SI, Grashkina IG, Yaksanova EM. For pathogenesis and therapy of rosacea and perioral dermatitis. Vestn Dermatologii Venerologii. 1980;(4):38-40. (In Russ.)
4. Adaskevich VP. Perioral dermatitis: clinical picture, diagnosis, treatment. Consilium medicum. 2008;(1):17-20. (In Russ.)
5. Araviyskaya ER, Sokolovskiy EV. Microbiome: a new era in the study of healthy and pathologically modified skin. Vestn Dermatologii Ve-nerologii. 2016;(3):102-9. (In Russ.)
6. Olisova OYu, Gromova SA. Perioral dermatitis. Rus Med Zhurn Dermatologiia. 2003;(17):972-5. (In Russ.)
7. Dolenc-Voljc M, Pohar M, Lunder T. Density of Demodex folliculorum in perioral dermatitis. Acta DermVenereol. 2005;85(3):211-5. doi: http://dx.doi.org/10.1080/00015550510030069
8. Schofer H. Rosazea: Klinik und aktuelle Therapie. Stuttgart: Thieme; 2003. 89 p.
9. Kogan BG, Stepanenko VL, Gorgol VT, Pavlyshin AV. Role of Demodex mites and Helicobacter infection in etiopathogenesis of rosacea, demodicoses, perioral dermatitis and acne disease. Eur Acad Dermatol Venerol. 2003;17(Suppl 3):165.
10. OOO NPF Litekh. Guidance on the use of dentoscrin kits for the detection of specific DNA fragments of periodontal pathogens by PCR with real-time fluorescence detection of the result (Real Time). Moscow, RF: LITEKh; 2017. 59 р. (In Russ.)
11. Zhizhin KS. Medical statistics: ucheb posobie. Rostov on Don, RF: Feniks; 2007. 160 р. (In Russ.)
12. Rebrova OYu. Statistical analysis of medical data. Application of STATISTICA application package. Moscow, RF: Media Sfera; 2002. 305 р.
13. Vorob’yev AA, Bykov AS, red. Atlas of medical microbiology, virology and immunology: ucheb posobie dlia studentov med vuzov. Moscow, RF: MIA; 2003. 236 р.
14. Buck A, Kalkoff KW. Zum Nachweis von Fusobakterien aus Effloreszenzen der perioralen Dermatitis. Hautarzt. 1971;22:433-6.
15. Berardi P, Benvenuti S, Genga A, Cecchini F. Demonstration of fusobacteria in eruptions of perioral dermatitis using the tape stripping toluidine blue method. J Eur Acad Dermatol Venereol. 1994;3(4):495-9.
16. Takiwaki H, Tsuda H, Arase S, Takeichi H. Differences between intrafollicular microorganism profiles in perioral and seborrhoeic dermatitis. Clin Exp Dermatol. 2003 Sep;28(5):531-4.
17. Maeda A, Ishiguro N, Kawashima M. The pathogenetic role of rod-shaped bacteria containing intracellular granules in the vellus hairs of a patient with perioral dermatitis: A comparison with perioral corticosteroid-induced rosacea. Australas J Dermatol. 2016 Aug;57(3):225-8. doi: http://dx.doi.org/10.1111/ajd.12344
Information about authors:
Adaskevich U.P. – Doctor of Medical Sciences, professor, head of the Chair of Dermatovenereology, Vitebsk State Order of Peoples’ Friendship Medical University;
Miadzelets M.O. – postgraduate of the Chair of Dermatovenerology, Vitebsk State Order of Peoples’ Friendship Medical University;
Stakhovich I.I. – head of the Clinicodiagnostic Laboratory, VSMU clinic;
Generalov I.I. – Doctor of Medical Sciences, professor, head of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Dermatovenereology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Maryiana O. Miadzelets.
