Menu

A+ A A-

Download article

DOI: https://doi.org/10.22263/2312-4156.2019.4.40

Goncharova A.I., Okulich V.K., Ziamko V.Y., Senkovich S.A.
Antimicrobial activity of lysozyme as a nonspecific resistance factor
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus

Vestnik VGMU. 2019;18(4):40-45.

Abstract.
Objectives. To study the modern etiological structure of sialadenitis with PCR diagnosis taken into account; to establish the role of lysozyme in the development of inflammatory diseases of the large salivary glands.
Material and methods. 86 patients with sialadenitis were examined. These patients underwent inpatient treatment in the Vitebsk Regional Clinical Hospital. In parallel with the bacteriological method of the study, multiplex PCR diagnosis was performed in real time. To detect DNA a set of reagents «Septoscreen» («Litech», Russia) was used. The result was evaluated by means of the program Bio Rad CFX Manager 3.0.
Results. During bacteriological studies, 70 isolates were isolated in 67 (77.9%) patients with sialadenitis, and inoculation negative results were obtained in 19 cases (22.1%). The modern etiological structure of sialadenitis was studied taking into consideration multiplex PCR diagnosis. Multiplex PCR diagnosis in comparison with the bacteriological method is by 16.6% more sensitive. During the experiment the antimicrobial activity of lysozyme against standard strains and isolates isolated from patients with inflammatory diseases of the large salivary glands was determined.
Conclusions. The modern etiological structure of sialadenitis has been studied with PCR diagnosis taken into account, the role of lysozyme in the development of inflammatory diseases of the large salivary glands has been established.
Key words: sialadenitis, inflammatory disease of the large salivary glands, PCR diagnosis, lysozyme.

References

1. Lamont RDzh, Lantts MS, Berne RA, Leblank DDzh, Leont'yev VK, red. Microbiology and immunology for dentists. Moscow, RF: Prakt meditsina; 2010. 504 р. (In Russ.)
2. Tsarev VN, red. Microbiology, virology and oral immunology: uchebnik. Moscow, RF: GEOTAR-Media; 2013. 576 р. (In Russ.)
3. Zelenova EG, Zaslavskaya MI, Salina EV, Rassanov SP. Oral microflora: normal and pathological: ucheb posobie. Nizhny Novgorod, RF: NGMA; 2004. 158 р. (In Russ.)
4. Dobson J, Wilson M. Sensitization of oral bacteria in biofilms to killing by light from a low-power laser. Arch Oral Biol. 1992 Nov;37(11):883-7. doi: http://dx.doi.org/10.1016/0003-9969(92)90058-g
5. O'Neill JF, Hope CK, Wilson M. Oral bacteria in multi-species biofilms can be killed by red light in the presence of toluidine blue. Lasers Surg Med. 2002;31(2):86-90. doi: http://dx.doi.org/10.1002/lsm.10087
6. Atshan SS, Shamsudin MN, Karunanidhi A, van Belkum A, Lung LT, Sekawi Z, et al. Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA). Infect Genet Evol. 2013 Aug;18:106-12. doi: http://dx.doi.org/10.1016/j.meegid.2013.05.002
7. Lopukhov LV, Eydel'shteyn MV. Polymerase chain reaction in clinical microbiological diagnosis. Klin Mikrobiolog Antimikrob Khimioterapiia. 2000;2(3):96-106. (In Russ.)
8. Kosinets AN, Struchkov YuV. Infection in surgery: rukovodstvo. Vitebsk, RB: VGMU; 2004. 510 р. (In Russ.)
9. Janeway CA, Travers P, Walport M, Shlomchik MJ. Immunobiology. 5th ed. New York: Garland Science; 2001.
10. Nazarenko GI, Kishkun AA. Clinical evaluation of laboratory results. Moscow, RF: Meditsina; 2002. 541 р. (In Russ.)
11. Jacobs DS, DeMott WR, Oxley DK. Laboratory test handbook. Hudson, Ohio: Lexi-comp; 2004. 1342 p.
12. Sanghamitra NJ, Ueno T. Expanding coordination chemistry from protein to protein assembly. Chem Commun (Camb). 2013 May;49(39):4114-26. doi: http://dx.doi.org/10.1039/c2cc36935d
13. Venkataramani S, Truntzer J, Coleman DR. Thermal stability of high concentration lysozyme across varying pH: A Fourier Transform Infrared study. J Pharm Bioallied Sci. 2013 Apr;5(2):148-53. doi: http://dx.doi.org/10.4103/0975-7406.111821
14. Goncharova AI. Biofilm forming microorganisms and oral fluid enzymes in the pathogenesis of siadenites. Vestn VGMU. 2018;17(3):58-66. (In Russ.)

Information about authors:
Goncharova A.I. – senior lecturer of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Okulich V.K. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-8226-6405;
Ziamko V.Y. – postgraduate of the Chair of Anesthesiology & Critical Care Medicine with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Senkovich S.A. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University.

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Clinical Microbiology. E-mail: – Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Goncharova Anna Igorevna.

Поиск по сайту