DOI: https://doi.org/10.22263/2312-4156.2019.4.74
Pabiarzhyn V.V.
Changes in the expression of markers in the glioma tissue of rats with ascariasis
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2019;18(4):74-80.
Abstract.
Objectives. To study the effect of Ascaris invasion in the dose of 40 Ascaris suum eggs per gram of animal body weight on the expression of glioma markers in the experiment, depending on the observation period.
Material and methods. The experiment was carried out on female Wistar rats and consisted of 2 experimental series. Experimental animals after modelling the tumor of rat C6 glioma in situ were divided into 8 groups of 10 animals each. The first series of the experiment included animals of groups 1-4 from which the material was taken on the 14th, 21st, 28th, 35th days of tumor development, respectively, to obtain the results in «clean» tumor of rat C6 glioma in situ. The second series included animals of groups 5-8, the study of their material was carried out to assess the impact of Ascaris invasion on the studied parameters depending on the time from the beginning of infection. The material of all groups of animals (tumor, the liver, the lungs, the brain) was used for macroscopic, histological, and immunohistochemical analyses with the purpose of determining glial fibrillar acid protein (GFAP), S 100, proliferative activity marker Ki-67.
Results. It has been revealed that A. suum invasion in the dose of 40 eggs per gram of animal body weight 2.16 times increases GFAP expression in the biopsy specimens of the tumor tissue of rat C6 glioma in situ by the 7th day of invasion development; the expression of S 100 by the 7th day of invasion development – 2.53 times, by the 14th day after infection – 2.05 times; the proliferative activity index Ki 67 by the 7th day of invasion development – 2.28 times, by the 14th day after infection – 2.22 times.
Conclusions. The obtained data show that Ascaris invasion in the dose of 40 eggs per 1 gram of animal body mass increases the expression of GFAP, S 100 and proliferative activity marker Ki-67 in the tissues of rat C6 glioma in situ during the period of high biological activity of the parasite.
Key words: rat, glioma, immunohistochemical markers, GFAP, S 100, Ki-67, ascariasis.
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Information about authors:
Pabiarzhyn V.V. – Candidate of Biological Sciences, associate professor, doctoral candidate of the Chair of Infectious Diseases, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Infectious Diseases. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Vyacheslav V. Pobyarzhin.
