Species composition of microorganisms being a part of microbiocenosis of the swimming-pools
Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Republic of Belarus
Vestnik VGMU. 2019;18(5):35-44.
Objectives. To determine species composition of microorganisms (MO), being a part of microbiocenosis of the swimming – pools (SP).
Material and methods. Water samples and swabs from the surfaces of the premises and technological equipment were taken from 9 SP. Sampling, transportation and analysis were provided according to active legislation. MO identification resulted from bacteriological survey and with the help of the analyzer Vitek 2 Compact (Biomerieux, France).
Results. 95 (46.1%) strains of gram-negative bacteria (GNB), 86 (41.7%) strains of gram-positive bacteria (GPB), 11 (5.3%) strains of molds, 8 (3.9%) strains of dermatophytes and 6 (2.9%) strains of yeast-like MO were isolated.
The majority of all strains were isolated from the walls in shower-baths (67 strains, 32.5%) and foot baths (61 strain, 29.6%), the minority were found in water samples taken from 25 cm depth (8 strains, 3.9%). The most frequent contamination level in the SP was 103 CFU/ml, 61% of MO were isolated in quantities of 103-107 CFU/ml.
The majority of GPB belonged to Staphylococcus spp. (40 strains, 46.5%). The part of S. aureus (13 strains) made up 32.5% of all staphylococci found. In the structure of GNB the group of non-fermentative bacteria (NGNB) prevailed (84 strains (88.4% of GNB)).
Conclusions. Low contamination levels of SP water cannot serve as an accurate and sufficient indicator of their microbiological safety. High levels of environmental contamination with MO create a pre-condition for SP visitors of getting an infection, which may lead to the increased quantity of infections, associated with the SP visits, especially among immunocompromised persons.
Key words: swimming-pools, contamination, environment, microorganisms.
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Information about authors:
Pugach V.V.– research officer of the Laboratory for Clinical and Experimental Microbiology, Republican Research and Practical Center for Epidemiology & Microbiology,