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DOI: https://doi.org/10.22263/2312-4156.2019.6.30

Miklis N.I., Adamenko G.V., Burak I.I.
Microbiological effectiveness of ethanol containing medicinal agents for preventive antisepsis
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus

Vestnik VGMU. 2019;18(6):30-36.

Abstract.
The aim of this work was to study the microbiological effectiveness of the developed «Vitasept» antiseptics against the spore-forming bacterium B.subtilis.
In the quantitative suspension test without protein loading the «Vitasept» antiseptic drugs caused a decrease in the number of B.subtilis ATCC 6633 test culture with RF from 6.0 lg to 6.61 lg on 1 min exposure. The reduction factor of 72% ethanol with respect to B. subtilis made up 5.93±0.1 lg.
In «Vitasept-SKZ», the reduction factor was 1.05 times (p<0.05), «Vitasept-SKZ-A» – 1.01 times (p>0.05), «Vitasept-SKI» – 1.04 times (p<0.01), «Vitasept-SKI-A» – 1.03 times (p>0.05), «Vitasept-SKO» – 1.11 (p<0,001), «Vitasept-SKO-A» – 1.06 (p<0.01) times, respectively, higher than that of 72% ethanol.
The study of the microbiological effectiveness of «Vitasept» antiseptics against the spore-forming B.subtilis ATCC 6633 by the disk-diffusion method showed that the B.subtilis ATCC 6633 growth free zone under the influence of 72% ethanol was 11.0±1.7 mm. Compared to 72% ethanol  the B.subtilis growth inhibition zone of «Vitasept-SKZ» was 1.6 times (p<0.001), «Vitasept-SKI» – 1.4 times (p<0.05), «Vitasept-SKO» – 1.8 times (p <0.001), «Vitasept-SKO-A» – 1.4 times (p<0.05) higher, «Vitasept-SKZ-A» and «Vitasept-SKI-A» did not significantly differ from 72% ethanol  (p>0.05).
The results of the conducted study allow us to conclude that the «Vitasept» antiseptics developed on the basis of 72% ethanol and 72% ethanol possess high microbiological effectiveness against the spore-forming B. subtilis ATCC 6633 in qualitative and quantitative suspension tests and meet the requirements of normative documentation.
The developed «Vitasept» antiseptic drugs containing environmentally friendly 72% ethanol of the brand de luxe: «Vitasept-SKZ», «Vitasept-SKZ-A», «Vitasept-SKI», «Vitasept-SKI-A», «Vitasept-SKO», «Vitasept-SKO-A» produced by «Bobruisk plant of biotechnologies», as well as 72% ethanol are characterized by high in vitro microbiological effectiveness against spore-forming B.subtilis.
Key words: antiseptic, «Vitasept», microbiological effectiveness, ethanol, preventive antisepsis.

The authors express their deep gratitude to the staff members of the Clinical Microbiology Chair and personally to its head, Professor Generalov I.I. for the afforded opportunity to conduct the investigation and for his counselling.

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Information about authors:
Miklis N.I. – Candidate of Medical Sciences, associate professor, head of the Chair of General Hygiene & Ecology, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-7707-5882;
Adamenko G.V. – senior lecturer of the Chair of Organization & Economy of Pharmacy with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Burak I.I. – Doctor of Medical Sciences, professor of the Chair of General Hygiene & Ecology, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-7204-3056.

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Organization & Economy of Pharmacy with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Gennadiy V. Adamenko.

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