DOI: https://doi.org/10.22263/2312-4156.2020.1.73
Zybleva S.V., Zyblev S.L.
The dynamics of CD3+CD4+CD8+ T-lymphocytes in kidney transplant recipients in the early and late post-transplantation period
Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus
Vestnik VGMU. 2020;19(1):73-79.
Abstract.
Objectives. To study the dynamics of the level of CD3+CD4+CD8+ T-lymphocytes on different course variants of the post-transplantation period in patients after kidney transplantation.
Material and methods. The study included 165 recipients who underwent kidney transplantation. Immunological examination was conducted before the transplantation and on the 3rd, 7th, 30th, 90th, 180th and 360th day after the transplantation. Four groups were formed: KTR1 – patients with satisfactory primary and late graft function, KTR2 –patients with satisfactory primary function and late dysfunction, KTR3 – those with primary dysfunction and satisfactory late function, KTR4 – patients with primary and late graft dysfunction. When creatinine levels were lower than 300 μmol/L on the 7th day, the function was considered as satisfactory primary one. Primary graft dysfunction was considered when creatinine values were equal to or higher than 300 μmol/L and when dialysis was necessary during the first week after transplantation. Satisfactory late graft function was characterized by the creatinine concentration after a year below 150 μmol/L, the absence of episodes of transplant rejection and dialysis during the first year.
Results. In KTR1 and KTR2 groups, there were no significant differences in CD3+CD4+CD8+ for 3 months. In these groups, a significant decrease of CD3+CD4+CD8+ was observed by the 7th day followed by an increase by the 30th day. Beginning with the 180th day, a significant increase in the level of CD3+CD4+CD8+ was observed in the KTR2 group compared to the KTR1 group. A significant prevalence of CD3+CD4+CD8+ was found in the KTR1 group before surgery and up to 3 months compared to the KTR3 and the KTR4 groups. Further, we observed a decrease in T-lymphocytes in the KTR3 and KTR4 groups. On the 180th day there were no significant differences between the KTR3, KTR4 and KTR1groups. One year after transplantation, the levels of CD3+CD4+CD8+ were significantly lower in the KTR1 and KTR3 groups than those in the KTR4 group.
Conclusions. Patients with satisfactory primary graft function are characterized by the decrease in the level of CD3+CD4+CD8+, which is not observed in the groups with primary graft dysfunction. Graft dysfunction in the late post-transplantation period is characterized by the increase in the CD3+CD4+CD8+ level compared to groups with satisfactory late graft function.
Key words: kidney transplantation, CD3+CD4+CD8+, renal graft dysfunction.
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Information about authors:
Zybleva S.V. – Candidate of Medical Sciences, academic secretary, immunologist, Republican Research Center for Radiation Medicine and Human Ecology;
ORCID: https://orcid.org/0000-0003-3061-5324
Zyblev S.L. – Candidate of Medical Sciences, associate professor, surgeon of the surgical department (transplantation, reconstructive and endocrine surgery), Republican Research Center for Radiation Medicine and Human Ecology,
ORCID: https://orcid.org/0000-0002-0968-6630
Correspondence address: Republic of Belarus, 246000, Gomel, 5 Ilicha str., Republican Research Center for Radiation Medicine and Human Ecology, Scientific Department. E-mail: zyb-svetlana@ yandex.by – Svetlana V. Zybleva.
