DOI: https://doi.org/10.22263/2312-4156.2020.3.23
Pashinskaya E.S., Semenov V.M., Pabiarzhyn V.V., Kubrakov K.M.
The reproduction technique of experimental metastatic rat glioma C6 with the use of a fluorescent dye
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(3):23-29.
Abstract.
The aim of this study is to develop a technique for reproducing experimental metastatic rat glioma C6 using a fluorescent dye.
The experiment was performed on 10 female Wistar rats. The goal was achieved by suspending a culture of C6 tumor cells in a culture medium, then adding a fluorescent CellBrite™ Orange Cytoplasmic Membrane Dye to the resulting suspension and placing it in an incubator. After incubating the C6 tumor cell suspension with CellBrite™ Orange Cytoplasmic Membrane Dye, it was removed and washed three times. The resulting suspension of colored living tumor cells in a concentration of 10x109 was injected subcutaneously into the supraclavicular region. The animal was then given an intramuscular injection of metpresone during 10 days. After 3 months at the site of introduction of colored tumor cells C6 culture neoplasm was fixed. The neoplasm and internal organs of the rat were taken to assess the migration of colored C6 tumor cells in order to confirm the metastasis process using a confocal microscope. Preparations from organ biopsies were prepared for immunohistochemical confirmation of gliomic specificity by determining GFAP and S-100 markers.
Key words: glioma, metastasis, fluorescent dye, rat.
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Information about authors:
Pashinskaya Е.S. – Candidate of Biological Sciences, associate professor, doctoral candidate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Semenov V.M. – Doctor of Medical Sciences, professor, head of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.
Pabiarzhyn V.V. – Candidate of Biological Sciences, associate professor, dean of the Overseas Students Training Faculty, Vitebsk State Order of Peoples’ Friendship Medical University;
Kubrakov K.M. – Candidate of Medical Sciences, associate professor of the Chair of Neurology & Neurosurgery, doctoral candidate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Еkaterina S. Pashinskaya.
