DOI: https://doi.org/10.22263/2312-4156.2020.4.107
Karazhan N.V.1, Sidoranka V.Y.2
The influence of Artemisia vulgaris herb on the course of allergic reactions in an experiment
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Republican Unitary Enterprise «Belfarmatsiya», Minsk, Republic of Belarus
Vestnik VGMU. 2020;19(4):107-112.
Abstract.
The aim of this research was to evaluate the effect of tea from Artemisia vulgaris herb on the course of simulated allergic reactions in in vivo and in vitro experiments.
The study was performed on outbred male mice in an anaphylactoid reaction model in vivo and mast cell membrane stabilization model in vitro. For sensitization in these models, compound 48/80 was used.
In the model of anaphylactoid reaction initiated by compound 48/80 in vivo, no animal death from the anaphylactoid reaction was observed in any of the studied groups. The severity of the anaphylactoid reaction depended on the dose of the studied medicine used in the group. An increase in the applied dose of tea from Artemisia vulgaris herb worsened the course of the anaphylactoid reaction. The Weigle index in the studied groups was 1.5-1.9 times higher than that in the placebo group.
On the model of stabilization of mast cell membranes in vitro, it was found that in the control with physiological saline in the placebo group, 78-86% of mast cells were without any signs of degranulation. The number of degranulated mast cells in the control of the studied groups of animals that received tea from Artemisia vulgaris herb was in all cases higher than in the control of the placebo group, but did not differ between the studied groups themselves.
When compound 48/80 was added to the mast cells in the studied groups of animals receiving tea at a dose of 200 and 400 mg/kg, a sharp increase in the percentage of degranulated mast cells up to 81.3±4.3% was observed. The percentage of degranulated mast cells in the group receiving tea at a dose of 100 mg/kg in the presence of compound 48/80 did not differ from that for the studied sample of the placebo group.
Thus, tea from Artemisia vulgaris herb worsens the course of simulated allergic reactions in in vivo and in vitro experiments. Proceeding from this, it is not recommended to use tea from Artemisia vulgaris herb to people suffering from allergic reactions.
Key words: Artemisia vulgaris herb, tea, anaphylactoid reaction, mast cells.
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Information about authors:
Karazhan N.V. – Candidate of Pharmaceutical Sciences, associate professor, dean of the Pharmaceutical Faculty, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0003-1924-717X,
Sidoranka V.Y. – pharmacist, Republican Unitary Enterprise «Belfarmatsiya».
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Pharmaceutical Faculty dean’s office. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Natallia V. Karazhan.
