DOI: https://doi.org/10.22263/2312-4156.2021.1.31
Asochuk S.S., Yakovleva O.S., Martsinkevich A.F., Karpenko E.A.
The dynamics of bone tissue morphologic changes in laboratory rats receiving atorvastatin and α-calcidol during three and six months
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2021;20(1):31-39.
Abstract.
Osteoporosis ranks fourth among the most significant human illnesses, after cardiovascular diseases, cancer and diabetes mellitus.
The aim of our work was to compare the effect of three- and six-month administration of atorvastatin and α-calcidol on the morphologic parameters of the laboratory rats bone tissue.
The experiment was carried out during 3 and 6 months on 240 laboratory rats divided into five groups: intact animals, «placebo» animals, animals with the introduction of atorvastatin alone, α-calcidol alone, atorvastatin and α-calcidol intragastrically. Euthanasia was performed under ether anesthesia. Pieces of the femur 0.5 cm in size were cut out in the proximal metaphysis area, and the branches of the mandibular bone were cut out in the diastema. The growth zone was impregnated with silver according to Lilly and stained according to Van Gieson.
The examination of histosections was carried out on the Olympus BX-40 microscope. The size of the newly formed bone tissue stained with silver salts in black was measured using the ImageScope-M software. The number of vessels was counted in 10 fields of view with the calculation of the average value. Statistical analysis of the data was carried out using the R 3.6.3 software package, taking into account compliance with the law of normal distribution and applying corrections for multiple studies. Differences were considered statistically significant at p-values <0.05.
As a result of the study the following conclusions have been made:
1. The growth zone of the femur bone tissue increases more after 6 months than after 3 months of the experiment.
2. The main changes in the studied parameters under the influence of the administered drugs occur already after 3 months and remain practically unchanged in comparison with 3 months to 6 months of the experiment.
Key words: bone tissue, α-calcidol, atorvastatin, mandibular bone, femur, osteoporosis, osteogenesis.
Sources of financing: The research was conducted within the frames of the theme task of State Research Programs (GPNI) «Basic and applied sciences to medicine» (№GR 20190142 of 26.02.2019).
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Information about authors:
Аsochuk S.S. – Doctor of Medical Sciences, professor, head of the Scientific-Research Laboratory, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0003-2074-3832
Yakovleva O.S. – senior lecturer of the Chair of Pediatric Dentistry & Orthodontics with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-6833-5005
Martsinkevich A.F. – Candidate of Biological Sciences, associate professor of the Chair of General & Clinical Biochemistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0003-3655-4489
Karpenko E.A. – Candidate of Veterinary Sciences, senior research officer of the Scientific-Research Laboratory, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-4099-8405
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Pediatric Dentistry & Orthodontics with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Olga S. Yakovleva.
