DOI: https://doi.org/10.22263/2312-4156.2021.1.40
Tkachenko A.S.1, Posokhov Y.A.1,2
The study of the phospholipid bilayer state of cell membranes in leukocytes of rats after oral consumption of E407a food additive
1Kharkiv National Medical University, Kharkiv, Ukraine
2National Technical University «Kharkiv Polytechnic Institute», Kharkiv, Ukraine
Vestnik VGMU. 2021;20(1):40-45.
Abstract.
Objectives. To assess the influence of orally administered semi-refined carrageenan (E407a) on the hydrophobic region of phospholipid bilayer in cell membranes of circulating white blood cells.
Material and methods. The fluorescent probe (2-phenyl-phenanthro[9,10-d]-1,3-oxazole) was used to evaluate the state of lipid bilayer in leukocytes obtained from 8 female adult WAG rats orally exposed to semi-refined carrageenan at a dose of 140 mg per kg of weight during 2 weeks, and 8 control animals.
Results. No noticeable changes in the shape of the probe fluorescence spectrum were observed in response to oral administration of semi-refined carrageenan. This indicates that oral intake of E407a causes no changes in the proton-donor ability of the medium in the lipid membranes of leukocytes in the region where the probe is located. A reduction in the emission intensity of the probe, attributed to the decrease in the number of the probe molecules bound to membranes during 1 h of incubation, was detected in the rats which were orally administered E407a food additive.
Conclusions. Oral consumption of semi-refined carrageenan doesn’t cause any changes in the membrane hydration of the hydrophobic region of leukocyte phospholipid bilayer.
Key words: fluorescent probes, membrane order, membrane hydration, white blood cells.
The study was conducted within the frames of the research work of Kharkiv National Medical University (Kharkiv, Ukraine) «Biochemical mechanisms of the intestine inflammation induction and means of its correction» (№GR 0120U102645).
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Information about authors:
Tkachenko A.S. – Candidate of Medical Sciences, associate professor, acting director of the Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University;
Posokhov Y.O. – Doctor of Chemical Sciences, senior lecturer of the Educational and Scientific Institute of Chemical Technology and Engineering, National Technical University «Kharkiv Polytechnic Institute».
Correspondence address: Ukraine, 661022, Kharkiv, 4 Nauky ave., Kharkiv National Medical University, Research Institute of Experimental and Clinical Medicine. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anton S. Tkachenko.
