DOI: https://doi.org/10.22263/2312-4156.2021.2.56
Ziamko V.Y.1, Okulich V.K.1, Dzyadzko A.M.2, Kolchanova N.E.1, Goncharova A.I.1, Kornilov A.V.1, Kabanava А.А.1, Averchankava N.A.3
The dependence of microorganisms biofilm formation on the features of the infectious process
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Minsk Scientific and Practical Center of Surgery, Transplantation and Hematology, Minsk, Republic of Belarus
3Ziegelberg Women’s Hospital, Aschaffenburg, Germany
Vestnik VGMU. 2021;20(2):56-64.
Abstract.
Objectives. To study the dependence of the formation intensity of microbial biofilms by isolates from patients on the severity and localization of the infectious process.
Material and methods. The study conducted from 2016 to 2020 included 894 clinical isolates from 720 patients with infectious pathology of various localization and severety. The formation intensity of microbial biofilms by isolates received from these patients has been studied, depending on the severity and localization of the infectious process.
Results. The property to form biofilms was found out in the overwhelming majority of tested isolates (86.6%). At the same time P. aeruginosa isolates had the maximum biofilm-forming ability, the weight of the microbial biofilm of which was 48.25; 30.1-70.2 mcg/well. The conducted studies have shown the dependence of biofilm formation on the severity of the disease in which isolates were isolated. A direct close relationship was found between the severity of the disease and the mass of microbial biofilm of P. aeruginosа and K. pneumoniae, a weak one – in Acinetobacter spp. (r=0.73, r=0.7 and r=0.35, p<0.05 accordingly).
Conclusions. It can be assumed that intensive formation of biofilms by clinical isolates is an important factor of the aggressiveness and severity of the infectious process. Moreover, the conducted study has shown the dependence of biofilm formation on the severity of the disease in which isolates were isolated.
Key words: biofilm, infections, P. aeruginosа, K. pneumoniae, Acinetobacter spp., S. aureus, Streptococcus spp., E. coli.
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Information about authors:
Ziamko V.Y. – Candidate of Medical Sciences, associate professor of the Chair of Anesthesiology and Resuscitation with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-6753-2074
Okulich V.K. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-8226-6405
Dzyadzko A.M. – Doctor of Medical Sciences, head of the Department of Anesthesiology and Resuscitation, Minsk Scientific and Practical Center of Surgery, Transplantation and Hematology,
ORCID: https://orcid.org/0000-0003-1965-1850
Kolchanova N.E. – Candidate of Medical Sciences, associate professor of the Chair of Restorative Dentistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-3071-1001
Goncharova A.I. – Candidate of Medical Sciences, associate professor of the Chair of Maxillofacial Surgery & Operative Dentistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-8226-6405
Kornilov A.V. – lecturer of the Chair of Hospital Surgery with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0001-8709-6639
Kabanava A.A. – Candidate of Medical Sciences, associate professor, head of the Chair of Maxillofacial Surgery & Operative Dentistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-0121-1139
Аverchankava N.A. – obstetrician-gynecologist in Ziegelberg Women’s Hospital, Aschaffenburg, Germany.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Anesthesiology and Resuscitation with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Viktoriya Y. Ziamko.
