DOI: https://doi.org/10.22263/2312-4156.2021.3.82
Sychik S.I., Hrynchak V.A., Lappo L.G., Shturych А.А., Lisovskaya G.V., Krymskaya T.P.
Risk assessment for employees using picolinafen-based herbicide
Scientific-Practical Centre of Hygiene, Minsk, Republic of Belarus
Vestnik VGMU. 2021;20(3):82-89.
Abstract.
The aim of this work is to conduct a hygienic risk assessment for workers when using a herbicide based on picolinafen and to study its cumulative properties in a subacute experiment on male white rats.
Material and methods. The object of the study was a herbicide based on picolinafen (CAS No. 37641-05-5). The content in the preparation is 21 g/l. The treatment was carried out by spraying wheat at a consumption rate of 3.5 l/ha and a working solution of 250 l/ha. The risk assessment for employees was carried out in accordance with the current technical regulatory legal acts. The toxic effect in a subacute experiment was studied with daily intragastric administration of picolinafen-based herbicide to male white rats for 28 days. At the end of the experiment the indices of the blood morphofunctional state, as well as those of hormonal and biochemical status were determined.
Results. In the field, when carrying out mechanical treatments in compliance with the regulations for the use of the drug, there was no excess of the hygienic standards of the active substance in the air of the working area and there was no deterioration in working conditions. The total risk of dermal and inhalation exposure for the workers made up 0.015 and did not exceed the permissible level. The introduction of the herbicide based on picolinafen in a subacute experiment to rats at doses of 50.0, 300.0 and 900.0 mg/kg does not contribute to the development of lethal outcomes. According to the classification this preparation belongs to the substances with weakly expressed cumulation. However, the effect of a dose of 900.0 mg/kg of the drug initiated a statistically significant decrease in the relative coefficients of the liver mass by 12.5%, an increase in the content of leukocytes and platelets by 15.7% and 15.5%, respectively, against the background of a decreased average volume of platelets by 18,6%.
Conclusions. The research results made it possible to evaluate the picolinafen-based herbicide as a drug with an acceptable risk for workers in the agro-industrial complex. Under the conditions of a subacute experiment, a dose of 300.0 mg/kg of the herbicide can be accepted as a threshold (inactive), however, additional experiments are required with the use of morphological research methods, as well as experiments with a longer exposure period and an increase in the levels of administered doses.
Key words: picolinafen, plant protection products, herbicide, hygienic risk assessment, argo-industrial complex, cumulation.
References
1. Tarmure S, Alexescu TG, Orasan O, Negrean V, Sitar-Taut AV, Coste SC, et al. Influence of pesticides on respiratory pathology - a literature review. Ann Agric Environ Med. 2020 Jun;27(2):194-200. doi: http://dx.doi.org/10.26444/aaem/121899
2. Iliukova II, Grynchak VA, Anisovich MV, Popel AA, Vasileva MM. Features of determining the equivalence of generic pesticide products. V: M-vo zdravookhraneniia Resp Belarus', Nauch-prakt tsentr gigieny; Sychik SI, red. Zdorov'e i okruzhaiushchaia sreda: sb nauch tr. Minsk, RB; 2016. Vyp 26. Р. 225-8. (In Russ.)
3. Choudri BS, Charabi Y, Al-Nasiri N, Al-Awadhi T. Pesticides and herbicides. Water Environ Res. 2020 Oct;92(10):1425-1432. doi: http://dx.doi.org/10.1002/wer.1380
4. Gun-Hwi Lee, Kyung-Chul Choi. Adverse effects of pesticides on the functions of immune system. Comp Biochem Physiol C Toxicol Pharmacol. 2020 Sep;235:108789. doi: http://dx.doi.org/10.1016/j.cbpc.2020.108789
5. Chao Li, Afruza Begum, Jinkai Xue. Analytical methods to analyze pesticides and herbicides. Water Environ Res. 2020 Oct;92(10):1770-1785. doi: http://dx.doi.org/10.1002/wer.1431
6. Соmmission Regulation (EU) No283/2013 of 1 March 2013 setting out the data requirements for active substances in accordance with Regulation (EC) 1107/2009 of the European Parliament and of the Council 2009 concerning the placing of plant protection products on the market. Official J Eur Union. 2013;(L93):1-84.
7. Sharma A, Shukla A, Attri K, Kumar M, Kumar P, Suttee A, et al. Global trends in pesticides: A looming threat and viable alternatives. Ecotoxicol Environ Saf. 2020 Sep;201:110812. doi: http://dx.doi.org/10.1016/j.ecoenv.2020.110812
8. European Food Safety Authority (EFSA); Anastassiadou M, Brancato A, Brocca D, Cabrera LC, De Lentdecker C, Erdos Z, et al. Evaluation of confirmatory data following the Article 12 MRL review for picolinafen. EFSA J. 2018 Nov;16(11):e05489. doi: http://dx.doi.org/10.2903/j.efsa.2018.5489
9. Jin-Young Lee, Sunwoo Park, Whasun Limm, Gwonhwa Song. Picolinafen exerts developmental toxicity via the suppression of oxidative stress and angiogenesis in zebrafish embryos. Pestic Biochem Physiol. 2021 Jan;171:104734. doi: http://dx.doi.org/10.1016/j.pestbp.2020.104734
10. Leon ME, Schinasi LH, Lebailly P, Freeman LE, Nordby K-C, Ferro G, et al. Pesticide use and risk of non-Hodgkin lymphoid malignancies in agricultural cohorts from France, Norway and the USA: a pooled analysis from the AGRICOH consortium. Int J Epidemiol. 2019 Oct;48(5):1519-1535. doi: http://dx.doi.org/10.1093/ije/dyz017
11. Dang HT, Malone JM, Gill G, Preston C. Cross-resistance to diflufenican and picolinafen and its inheritance in oriental mustard (Sisymbrium orientale L.). Pest Manag Sci. 2019 Jan;75(1):195-203. doi: http://dx.doi.org/10.1002/ps.5087
12. Commission implementing regulation (EU) No 400/2014 of 22 April 2014 concerning a coordinated multiannual control programme of the Union for 2015, 2016 and 2017 to ensure compliance with maximum residue levels of pesticides and to assess the consumer exposure to pesticide residues in and on food of plant and animal origin. Official J Eur Union. 2014;(L119):44-55.
13. Iliukova II, Klochkova OP. Method for determining the health risk of workers when using pesticides: instruktsiia po primeneniiu № 008-0915: utv 07.04.2016 g. Minsk, RB; 2015. 16 р. (In Russ.)
14. Iurkevich ES, Iliukova II, Boris OA, Grynchak VA. Determination of toxicity of chemical products (chemicals and their mixtures) with repeated and chronic intragastric intake: instruktsiia po primeneniiu № 052-1215: utv. 30.08.2016 g. Minsk, RB; 2016. 28 р. (In Russ.)
15. Good Laboratory Practice = Good Laboratory Practice: TKP 125–2008. Vved 01.05.2008. Izd ofits. Minsk, RB: M-vo zdravookhraneniia Resp Belarus'; 2008. 34 р. (In Russ.)
16. Grynchak VA, Iliukova II, Sychik SI. Scientific substantiation of hygienic regulations for the content of picolinafen in the human environment. Toksikol Vestn. 2019;(6):50-5. (In Russ.)
17. Medved LI, Kagan IuS, Spynu EI. Pesticides and health problems. Zhurn Vsesoiuz Khim O-va DI Mendeleeva. 1968;13(3):263-71. (In Russ.)
Information about authors:
Sychik S.I. – Candidate of Medical Sciences, associate professor, director of the Scientific-Practical Centre of Hygiene,
ORCID: https://orcid.org/0000-0002-5493-9799
HrynchakV.A. – Candidate of Medical Sciences, head of the laboratory of applied toxicology and safety of medical devices, Scientific-Practical Centre of Hygiene;
ORCID: https://orcid.org/0000-0002-4119-1793
Lappo L.G. – associate research officer of the laboratory of applied toxicology and safety of medical devices, Scientific-Practical Centre of Hygiene;
Shturych А.А. – associate research officer of the laboratory of applied toxicology and safety of medical devices, Scientific-Practical Centre of Hygiene;
Lisovskaya G.V. – senior research officer of the laboratory of applied toxicology and safety of medical devices, Scientific-Practical Centre of Hygiene;
Krymskaya T.P. – head of the laboratory of chromatographic studies, Scientific-Practical Centre of Hygiene.
Correspondence address: Republic of Belarus, 220012, Minsk, 8 Akademicheskaya str., Scientific-Practical Centre of Hygiene. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Vitaly A. Grynchak.
