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DOI: https://doi.org/10.22263/2312-4156.2022.1.58

Rozhin V.V.¹, Chueshova N.V.², Nadyrov E.A.³, Kirilenko S.I.¹, Matveyenkau M.V.², Nikolaev V.I.³
Cell survival investigation of two types of autological osteoplastic materials in spine fusion procedures
¹Gomel Regional Clinical Hospital, Gomel, Republic of Belarus
²Institute of Radiobiology of the National Academy of Sciences of Belarus, Gomel, Republic of Belarus
³Gomel State Medical University, Gomel, Republic of Belarus

Vestnik VGMU. 2022;21(1):58-64.

Abstract.
Objectives. To compare the survival rate of «bone chip» cells and native transplant automixture by evaluating the architectonics of cell membranes.
Material and methods. Autograft in the form of a native graft automixture obtained using a «bone dust filtering device» and «bone chips» obtained using Kerrison rongeurs in 6 patients operated for degenerative diseases of the spine. The study was carried out using a flow cytometer by assessing the architectonics of cell membranes during the first three days.
Results. The study of native transplant automixture and «bone chips» determined the dispersed state of the first graft and the latter, similar in its structure to the whole bone. The spectrograms analysis showed a heterogeneous composition of grafts, and in the case of native transplant automixture, the scatter was more pronounced. The increase in the number of cells during the first three days of the native transplant automixture is probably associated with the elimination of contact inhibition of their proliferation and the release of osteoinductive factors from the bone matrix. The number of necrotic cells was higher in the native transplant automixture, which is due to the rough method of its preparation, however, this number was not statistically significant compared to the number of necrotic cells in the «bone chips». Despite the combined thermal and mechanical impact, the survival of the cell component in both cases made up more than 98.0% without any statistical significance in comparison between the two types of transplants (p>0.05).
Conclusions. The high survival rate of the cellular component of both types of autografts indicates that the osteogenic potential does not depend on the method of their preparation.
Key words: spondylodesis, cell survival, autograft, native graft automixture, «bone chips», architectonics, degenerative diseases of the spine.

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Information about authors:
Rozhin V.V. – neurosurgeon of the neurosurgical department No. 2, Gomel Regional Clinical Hospital,
ORCID: https://orcid.org/0000-0002-3022-6129
Chueshova N.V. – Candidate of Biological Sciences, head of the Department of Biological Systems Sustainability, Institute of Radiobiology of the National Academy of Sciences of Belarus;
Nadyrov E.A. – Candidate of Medical Sciences, associate professor of the Chair of Pathological Anatomy, Gomel State Medical University;
Kirilenko S.I. – Candidate of Medical Sciences, head of the neurosurgical department No. 2, Gomel Regional Clinical Hospital;
Matveyenkau M.V. – research officer of the Department of Biological Systems Sustainability, Institute of Radiobiology of the National Academy of Sciences of Belarus;
Nikolaev V.I. – Candidate of Medical Sciences, associate professor of the Chair of Traumatology, Orthopedics, Military Field Surgery, Gomel State Medical University,
ORCID: https://orcid.org/0000-0001-9886-7216

Correspondence address: Republic of Belarus, 246029, Gomel, 5 Brothers Lizyukov str., Gomel Regional Clinical Hospital, neurosurgical department No. 2. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Vladimir V. Rozhin.

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