DOI: https://doi.org/10.22263/2312-4156.2022.6.47
A.-M.V. Yerofeyeva
The impact of pharmacological blocking of type 1cannabinoid receptors on the effectiveness of mesenchymal stem cell transplantation in experimental peripheral neuropathy
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk, Republic of Belarus
Vestnik VGMU. 2022;21(6):47-56.
Abstract.
Objectives. To study the impact of pharmacological blockade of type 1cannabinoid receptors (СВ1) located on adipose-derived mesenchymal stem cells (ADMSCs) or perineural tissue in the area of sciatic nerve axotomy on anti-nociceptive actions of local stem cell transplantation in experiment.
Material and methods. Neuropathic pain was induced in Wistar rats (n=57) by the full sciatic nerve axotomy in the left hind paw. Pharmacological blockade of CB1 receptors was done on ADMSCs in rats or their hind paw tissues in the surgery area and then stem cells were transplanted at a dose of 1x106 cells/kg to the site of sciatic nerve axotomy. Nociceptive responses to mechanical and thermal stimuli were assessed in dynamics, as well as detailed gait analysis was made with the help of CatWalk XT.
Results. It has been found that blocking of CB1 receptors of perineural tissues did not affect nociceptive reactions to mechanical and thermal stimuli and gait parameters compared to ADMSCs transplantation only. Nevertheless blockade of CB1 receptors on ADMSCs led to a more rapid development of the analgesic effect after stem cell transplantation, but at the same time, at the later stages of the experiment, the anti-nociceptive efficacy of ADMSCs was reduced and lasted for less time.
Conclusions. The obtained results testify to the involvement of CB1 receptors located on ADMSCs in anti-nociceptive actions after their transplantation to the area of peripheral nerve injury. For a better disclosure of the mechanisms of ADMSCs actions through CB1 receptors further investigations are needed.
Keywords: mesenchymal stem cells, neuropathic pain, sciatic nerve, cannabinoid receptors, sciatic functional index, gait analysis, hyperalgesia.
Ethical aspects. The approval of the Ethics Committee. The study has been approved by the Comission on Bioethics at the State Scientific Institution “Institute of Physiology of the National Academy of Sciences of Belarus”, minutes No.1 dated February 2, 2022.
Acknowledgements. The author expresses her deep gratitude to the scientific advisor, Candidate of Biological Sciences Molchanova A.Yu. for advisory assistance in preparation the manuscript of this article, as well as to the leading research officer of the Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Candidate of Biological Sciences Pinchuk S.V. for his assistance in isolating and preparing mesenchymal stem cells of the adipose tissue for transplantation.
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Information about authors:
A.-M.V. Yerofeyeva – postgraduate, associate research officer, Institute of Physiology of the National Academy of Sciences of Belarus, https://orcid.org/0000-0002-9407-9295
E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anna-Maria V. Yerofeyeva.
